Abstract
In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
| Original language | English |
|---|---|
| Pages (from-to) | 2725-2729 |
| Number of pages | 5 |
| Journal | Blood |
| Volume | 124 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 23 Oct 2014 |
| Externally published | Yes |
Cite this
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A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. / Potts, Kathryn S. ; Sargeant, Tobias J; Markham, John F. ; Shi, Wei; Biben, Christine; Josefsson, Emma C.; Whitehead, Lachlan W.; Rogers, Kelly L.; Liakhovitskaia, Anna ; Smyth, Gordon K.; Kile, Benjamin T.; Medvinsky, Alexander ; Alexander, Warren S. ; Hilton, Douglas J.; Taoudi, Samir.
In: Blood, Vol. 124, No. 17, 23.10.2014, p. 2725-2729.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo
AU - Potts, Kathryn S.
AU - Sargeant, Tobias J
AU - Markham, John F.
AU - Shi, Wei
AU - Biben, Christine
AU - Josefsson, Emma C.
AU - Whitehead, Lachlan W.
AU - Rogers, Kelly L.
AU - Liakhovitskaia, Anna
AU - Smyth, Gordon K.
AU - Kile, Benjamin T.
AU - Medvinsky, Alexander
AU - Alexander, Warren S.
AU - Hilton, Douglas J.
AU - Taoudi, Samir
PY - 2014/10/23
Y1 - 2014/10/23
N2 - In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
AB - In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
UR - http://www.scopus.com/inward/record.url?scp=84908242967&partnerID=8YFLogxK
U2 - 10.1182/blood-2014-02-559468
DO - 10.1182/blood-2014-02-559468
M3 - Article
VL - 124
SP - 2725
EP - 2729
JO - Blood
JF - Blood
SN - 0006-4971
IS - 17
ER -