A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo

Kathryn S. Potts, Tobias J Sargeant, John F. Markham, Wei Shi, Christine Biben, Emma C. Josefsson, Lachlan W. Whitehead, Kelly L. Rogers, Anna Liakhovitskaia, Gordon K. Smyth, Benjamin T. Kile, Alexander Medvinsky, Warren S. Alexander, Douglas J. Hilton, Samir Taoudi

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.

Original languageEnglish
Pages (from-to)2725-2729
Number of pages5
JournalBlood
Volume124
Issue number17
DOIs
Publication statusPublished - 23 Oct 2014
Externally publishedYes

Cite this

Potts, K. S., Sargeant, T. J., Markham, J. F., Shi, W., Biben, C., Josefsson, E. C., ... Taoudi, S. (2014). A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. Blood, 124(17), 2725-2729. https://doi.org/10.1182/blood-2014-02-559468
Potts, Kathryn S. ; Sargeant, Tobias J ; Markham, John F. ; Shi, Wei ; Biben, Christine ; Josefsson, Emma C. ; Whitehead, Lachlan W. ; Rogers, Kelly L. ; Liakhovitskaia, Anna ; Smyth, Gordon K. ; Kile, Benjamin T. ; Medvinsky, Alexander ; Alexander, Warren S. ; Hilton, Douglas J. ; Taoudi, Samir. / A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. In: Blood. 2014 ; Vol. 124, No. 17. pp. 2725-2729.
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title = "A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo",
abstract = "In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.",
author = "Potts, {Kathryn S.} and Sargeant, {Tobias J} and Markham, {John F.} and Wei Shi and Christine Biben and Josefsson, {Emma C.} and Whitehead, {Lachlan W.} and Rogers, {Kelly L.} and Anna Liakhovitskaia and Smyth, {Gordon K.} and Kile, {Benjamin T.} and Alexander Medvinsky and Alexander, {Warren S.} and Hilton, {Douglas J.} and Samir Taoudi",
year = "2014",
month = "10",
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doi = "10.1182/blood-2014-02-559468",
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pages = "2725--2729",
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Potts, KS, Sargeant, TJ, Markham, JF, Shi, W, Biben, C, Josefsson, EC, Whitehead, LW, Rogers, KL, Liakhovitskaia, A, Smyth, GK, Kile, BT, Medvinsky, A, Alexander, WS, Hilton, DJ & Taoudi, S 2014, 'A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo' Blood, vol. 124, no. 17, pp. 2725-2729. https://doi.org/10.1182/blood-2014-02-559468

A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. / Potts, Kathryn S. ; Sargeant, Tobias J; Markham, John F. ; Shi, Wei; Biben, Christine; Josefsson, Emma C.; Whitehead, Lachlan W.; Rogers, Kelly L.; Liakhovitskaia, Anna ; Smyth, Gordon K.; Kile, Benjamin T.; Medvinsky, Alexander ; Alexander, Warren S. ; Hilton, Douglas J.; Taoudi, Samir.

In: Blood, Vol. 124, No. 17, 23.10.2014, p. 2725-2729.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Potts, Kathryn S.

AU - Sargeant, Tobias J

AU - Markham, John F.

AU - Shi, Wei

AU - Biben, Christine

AU - Josefsson, Emma C.

AU - Whitehead, Lachlan W.

AU - Rogers, Kelly L.

AU - Liakhovitskaia, Anna

AU - Smyth, Gordon K.

AU - Kile, Benjamin T.

AU - Medvinsky, Alexander

AU - Alexander, Warren S.

AU - Hilton, Douglas J.

AU - Taoudi, Samir

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Potts KS, Sargeant TJ, Markham JF, Shi W, Biben C, Josefsson EC et al. A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. Blood. 2014 Oct 23;124(17):2725-2729. https://doi.org/10.1182/blood-2014-02-559468