TY - JOUR
T1 - A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo
AU - Potts, Kathryn S.
AU - Sargeant, Tobias J
AU - Markham, John F.
AU - Shi, Wei
AU - Biben, Christine
AU - Josefsson, Emma C.
AU - Whitehead, Lachlan W.
AU - Rogers, Kelly L.
AU - Liakhovitskaia, Anna
AU - Smyth, Gordon K.
AU - Kile, Benjamin T.
AU - Medvinsky, Alexander
AU - Alexander, Warren S.
AU - Hilton, Douglas J.
AU - Taoudi, Samir
PY - 2014/10/23
Y1 - 2014/10/23
N2 - In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
AB - In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.
UR - http://www.scopus.com/inward/record.url?scp=84908242967&partnerID=8YFLogxK
U2 - 10.1182/blood-2014-02-559468
DO - 10.1182/blood-2014-02-559468
M3 - Article
C2 - 25079356
AN - SCOPUS:84908242967
VL - 124
SP - 2725
EP - 2729
JO - Blood
JF - Blood
SN - 0006-4971
IS - 17
ER -