A large proportion of bovine T cells express the γδ T cell receptor and show a distinct tissue distribution and surface phenotype

Charles R. Mackay, Wayne R. Hein

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Abstract

The numbers, phenotype, and tissue distribution of γδ T cells in cattle were studied using two monoclonal antibodies (mAbs) which react with the bovine γδ T cell receptor (TCR). Both mAbs stained 20-40% of T cells in peripheral blood, and immunoprecipitated molecules of 44 and 36 kd (reduced) and 70-80 kd (non-reduced). In cattle the majority of circulating γδ T cells showed a distinct surface phenotype; they expressed T19, a 215 kd molecule described in sheep and cattle which marks only γδ T cells. Bovine γδ T cells were also CD2-, CD4-, and mostly CD8-, and falled to express CD6, a molecule possibly involved in T cell activation. The distribution of γδ T cells in cattle lymphoid tissues differed markedly from that in humans, in that bovine γδ T cells were concentrated around lymph node trabeculae and were usually sparse or absent from the B cell and T cell domains of lymph nodes. Like most other species studied, γδ T cells in cattle were localized to epithelial surfaces, particularly within the skin and intestine, indicating that it was at these sites where γδ T cells functioned. Our results provide further evidence for the unusual localization, recirculation pattern, and phenotype of γδ T cells, and also show that some features of γδ T cells can differ quite markedly from species to species.

Original languageEnglish
Pages (from-to)540-545
Number of pages6
JournalInternational Immunology
Volume1
Issue number5
DOIs
Publication statusPublished - 1 Nov 1989
Externally publishedYes

Keywords

  • γδ T cells
  • Surface phenotype
  • Tissue distribution

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