A label-free optical biosensor based on nanoporous anodic alumina for tumour necrosis factor-alpha detection in chronic wounds

Gayathri Rajeev, Elisabet Xifre-Perez, Beatriz Prieto Simon, Allison J. Cowin, Lluis F. Marsal, Nicolas H. Voelcker

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

Tumour necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine important to wound healing. In non-healing wounds, it has been suggested that the expression of TNF-α is prolonged and elevated which contributes to impaired healing responses. Hence it is of great interest to develop biosensors towards the detection of TNF-α in non-healing wounds. In this study, we have developed a label-free optical TNF-α biosensor based on interferometric reflectance spectroscopy (IRS) technique using a functionalized nanoporous anodic alumina (NAA) thin film transducer. The biosensor is fabricated by functionalizing NAA pore walls with anti-TNF-α antibodies using silanization chemistry. Binding of TNF-α to the bioreceptors within the pores causes a change in effective optical thickness (EOT) of the NAA thin film. Thus, analyte detection is achieved by monitoring EOT evolution with time. Label-free detection of TNF-α was demonstrated in buffer solution and in complex media such as simulated wound fluid. A limit of detection of 0.13 μg/mL was achieved. This study provides proof-of-concept evidence which sets foundations for further development of biosensors as point-of-care (POC) diagnostic tools for chronic wound care.

Original languageEnglish
Pages (from-to)116-123
Number of pages8
JournalSensors and Actuators B: Chemical
Volume257
DOIs
Publication statusPublished - 1 Mar 2018

Keywords

  • Chronic wound
  • Optical biosensor
  • Porous alumina biosensor
  • TNF-α detection

Cite this

@article{1b071f752a9c447684838001d8d2ac2a,
title = "A label-free optical biosensor based on nanoporous anodic alumina for tumour necrosis factor-alpha detection in chronic wounds",
abstract = "Tumour necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine important to wound healing. In non-healing wounds, it has been suggested that the expression of TNF-α is prolonged and elevated which contributes to impaired healing responses. Hence it is of great interest to develop biosensors towards the detection of TNF-α in non-healing wounds. In this study, we have developed a label-free optical TNF-α biosensor based on interferometric reflectance spectroscopy (IRS) technique using a functionalized nanoporous anodic alumina (NAA) thin film transducer. The biosensor is fabricated by functionalizing NAA pore walls with anti-TNF-α antibodies using silanization chemistry. Binding of TNF-α to the bioreceptors within the pores causes a change in effective optical thickness (EOT) of the NAA thin film. Thus, analyte detection is achieved by monitoring EOT evolution with time. Label-free detection of TNF-α was demonstrated in buffer solution and in complex media such as simulated wound fluid. A limit of detection of 0.13 μg/mL was achieved. This study provides proof-of-concept evidence which sets foundations for further development of biosensors as point-of-care (POC) diagnostic tools for chronic wound care.",
keywords = "Chronic wound, Optical biosensor, Porous alumina biosensor, TNF-α detection",
author = "Gayathri Rajeev and Elisabet Xifre-Perez and {Prieto Simon}, Beatriz and Cowin, {Allison J.} and Marsal, {Lluis F.} and Voelcker, {Nicolas H.}",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.snb.2017.10.156",
language = "English",
volume = "257",
pages = "116--123",
journal = "Sensors and Actuators B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

A label-free optical biosensor based on nanoporous anodic alumina for tumour necrosis factor-alpha detection in chronic wounds. / Rajeev, Gayathri; Xifre-Perez, Elisabet; Prieto Simon, Beatriz; Cowin, Allison J.; Marsal, Lluis F.; Voelcker, Nicolas H.

In: Sensors and Actuators B: Chemical, Vol. 257, 01.03.2018, p. 116-123.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A label-free optical biosensor based on nanoporous anodic alumina for tumour necrosis factor-alpha detection in chronic wounds

AU - Rajeev, Gayathri

AU - Xifre-Perez, Elisabet

AU - Prieto Simon, Beatriz

AU - Cowin, Allison J.

AU - Marsal, Lluis F.

AU - Voelcker, Nicolas H.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Tumour necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine important to wound healing. In non-healing wounds, it has been suggested that the expression of TNF-α is prolonged and elevated which contributes to impaired healing responses. Hence it is of great interest to develop biosensors towards the detection of TNF-α in non-healing wounds. In this study, we have developed a label-free optical TNF-α biosensor based on interferometric reflectance spectroscopy (IRS) technique using a functionalized nanoporous anodic alumina (NAA) thin film transducer. The biosensor is fabricated by functionalizing NAA pore walls with anti-TNF-α antibodies using silanization chemistry. Binding of TNF-α to the bioreceptors within the pores causes a change in effective optical thickness (EOT) of the NAA thin film. Thus, analyte detection is achieved by monitoring EOT evolution with time. Label-free detection of TNF-α was demonstrated in buffer solution and in complex media such as simulated wound fluid. A limit of detection of 0.13 μg/mL was achieved. This study provides proof-of-concept evidence which sets foundations for further development of biosensors as point-of-care (POC) diagnostic tools for chronic wound care.

AB - Tumour necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine important to wound healing. In non-healing wounds, it has been suggested that the expression of TNF-α is prolonged and elevated which contributes to impaired healing responses. Hence it is of great interest to develop biosensors towards the detection of TNF-α in non-healing wounds. In this study, we have developed a label-free optical TNF-α biosensor based on interferometric reflectance spectroscopy (IRS) technique using a functionalized nanoporous anodic alumina (NAA) thin film transducer. The biosensor is fabricated by functionalizing NAA pore walls with anti-TNF-α antibodies using silanization chemistry. Binding of TNF-α to the bioreceptors within the pores causes a change in effective optical thickness (EOT) of the NAA thin film. Thus, analyte detection is achieved by monitoring EOT evolution with time. Label-free detection of TNF-α was demonstrated in buffer solution and in complex media such as simulated wound fluid. A limit of detection of 0.13 μg/mL was achieved. This study provides proof-of-concept evidence which sets foundations for further development of biosensors as point-of-care (POC) diagnostic tools for chronic wound care.

KW - Chronic wound

KW - Optical biosensor

KW - Porous alumina biosensor

KW - TNF-α detection

UR - http://www.scopus.com/inward/record.url?scp=85032945981&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2017.10.156

DO - 10.1016/j.snb.2017.10.156

M3 - Article

AN - SCOPUS:85032945981

VL - 257

SP - 116

EP - 123

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

ER -