A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA

Wanhe Wang; Tian-Shu Kang; Philip Wai Hong Chan; Jin-Jian Lu; Xiu-Ping Chen; Chung-Hang Duncan Leung; Dik-Lung Edmond Ma

doi:10.1088/1468-6996/16/6/065004

A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA

Wanhe Wang, Tian-Shu Kang, Philip Wai Hong Chan, Jin-Jian Lu, Xiu-Ping Chen, Chung-Hang Duncan Leung, Dik-Lung Edmond Ma

School of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

22 Citations (Scopus)

Abstract

We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This ‘mix-and-detect’ methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Science and Technology of Advanced Materials
Volume	16
Issue number	6
DOIs	https://doi.org/10.1088/1468-6996/16/6/065004
Publication status	Published - 2015

Keywords

iridium(III)
exonuclease III
G-quadruplex
mercury(II) ion

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10.1088/1468-6996/16/6/065004

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abstract = "We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This {\textquoteleft}mix-and-detect{\textquoteright} methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+",

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T1 - A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA

AU - Wang, Wanhe

AU - Kang, Tian-Shu

AU - Chan, Philip Wai Hong

AU - Lu, Jin-Jian

AU - Chen, Xiu-Ping

AU - Leung, Chung-Hang Duncan

AU - Ma, Dik-Lung Edmond

PY - 2015

Y1 - 2015

N2 - We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This ‘mix-and-detect’ methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+

AB - We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This ‘mix-and-detect’ methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+

KW - iridium(III)

KW - exonuclease III

KW - G-quadruplex

KW - mercury(II) ion

UR - http://iopscience.iop.org.ezproxy.lib.monash.edu.au/article/10.1088/1468-6996/16/6/065004/pdf

U2 - 10.1088/1468-6996/16/6/065004

DO - 10.1088/1468-6996/16/6/065004

M3 - Article

SN - 1468-6996

VL - 16

SP - 1

EP - 6

JO - Science and Technology of Advanced Materials

JF - Science and Technology of Advanced Materials

IS - 6

ER -

A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA

Abstract

Keywords

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