A label-free G-quadruplex-based mercury detection assay employing the exonuclease III-mediated cleavage of T-Hg2+-T mismatched DNA

Wanhe Wang, Tian-Shu Kang, Philip Wai Hong Chan, Jin-Jian Lu, Xiu-Ping Chen, Chung-Hang Duncan Leung, Dik-Lung Edmond Ma

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22 Citations (Scopus)


We report herein the use of an exonuclease III and G-quadruplex probe to construct a G-quadruplex-based luminescence detection platform for Hg2+. Unlike common DNA-based Hg2+ detection methods, when using the dsDNA probe to monitor the hairpin formation, the intercalation of the dsDNA probe may be influenced by the distortion of dsDNA. This ‘mix-and-detect’ methodology utilized the G-quadruplex probe as the signal transducer and is simple, rapid, convenient to use and can detect down to 20 nM of Hg2+
Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalScience and Technology of Advanced Materials
Issue number6
Publication statusPublished - 2015


  • iridium(III)
  • exonuclease III
  • G-quadruplex
  • mercury(II) ion

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