A High Yield Method For The Isolation Of Sheep'S Liver Cathepsin L

Robert Pike; Clive Dennison

doi:10.1080/10826068908544913

A High Yield Method For The Isolation Of Sheep'S Liver Cathepsin L

Robert Pike, Clive Dennison

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Abstract

A method, giving twice the yield of the previous method¹for the isolation of sheep's liver cathepsin L is described. The method uses three phase partitioning (TPP) in t-butanol/water/ammonium sulphate mixtures, followed by two chromatographic steps, at different pH values, in a single column of S-SepharOse.

Original language	English
Pages (from-to)	231-245
Number of pages	15
Journal	Preparative Biochemistry
Volume	19
Issue number	3
DOIs	https://doi.org/10.1080/10826068908544913
Publication status	Published - 1 Jan 1989
Externally published	Yes

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title = "A High Yield Method For The Isolation Of Sheep'S Liver Cathepsin L",

abstract = "A method, giving twice the yield of the previous method1for the isolation of sheep's liver cathepsin L is described. The method uses three phase partitioning (TPP) in t-butanol/water/ammonium sulphate mixtures, followed by two chromatographic steps, at different pH values, in a single column of S-SepharOse.",

author = "Robert Pike and Clive Dennison",

year = "1989",

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N2 - A method, giving twice the yield of the previous method1for the isolation of sheep's liver cathepsin L is described. The method uses three phase partitioning (TPP) in t-butanol/water/ammonium sulphate mixtures, followed by two chromatographic steps, at different pH values, in a single column of S-SepharOse.

AB - A method, giving twice the yield of the previous method1for the isolation of sheep's liver cathepsin L is described. The method uses three phase partitioning (TPP) in t-butanol/water/ammonium sulphate mixtures, followed by two chromatographic steps, at different pH values, in a single column of S-SepharOse.

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U2 - 10.1080/10826068908544913

DO - 10.1080/10826068908544913

M3 - Article

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AN - SCOPUS:0024792668

SN - 0032-7484

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SP - 231

EP - 245

JO - Preparative Biochemistry

JF - Preparative Biochemistry

IS - 3

ER -

A High Yield Method For The Isolation Of Sheep'S Liver Cathepsin L

Abstract

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