A High Yield Method For The Isolation Of Sheep'S Liver Cathepsin L

Robert Pike, Clive Dennison

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Abstract

A method, giving twice the yield of the previous method1for the isolation of sheep's liver cathepsin L is described. The method uses three phase partitioning (TPP) in t-butanol/water/ammonium sulphate mixtures, followed by two chromatographic steps, at different pH values, in a single column of S-SepharOse.

Original languageEnglish
Pages (from-to)231-245
Number of pages15
JournalPreparative Biochemistry
Volume19
Issue number3
DOIs
Publication statusPublished - 1 Jan 1989
Externally publishedYes

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