Abstract
Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20 µm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labeled sections. Systematic in-vivo MRI of the individual animals facilitates injection placement into reference-atlas defined anatomical compartments. Further, by combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to accurately map individual brains into a common reference atlas despite the significant individual variation. This approach will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain.
| Original language | English |
|---|---|
| Article number | e40042 |
| Number of pages | 36 |
| Journal | eLife |
| Volume | 8 |
| DOIs | |
| Publication status | Published - 5 Feb 2019 |
Keywords
- annotation
- high-throughput pipeline
- marmoset
- mesoscale
- neuroscience
- registration
- tape-transfer method
Cite this
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A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset. / Lin, Meng Kuan; Takahashi, Yeonsook Shin; Huo, Bing Xing; Hanada, Mitsutoshi; Nagashima, Jaimi; Hata, Junichi; Tolpygo, Alexander S.; Ram, Keerthi; Lee, Brian C.; Miller, Michael I.; Rosa, Marcello GP; Sasaki, Erika; Iriki, Atsushi; Okano, Hideyuki; Mitra, Partha.
In: eLife, Vol. 8, e40042, 05.02.2019.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset
AU - Lin, Meng Kuan
AU - Takahashi, Yeonsook Shin
AU - Huo, Bing Xing
AU - Hanada, Mitsutoshi
AU - Nagashima, Jaimi
AU - Hata, Junichi
AU - Tolpygo, Alexander S.
AU - Ram, Keerthi
AU - Lee, Brian C.
AU - Miller, Michael I.
AU - Rosa, Marcello GP
AU - Sasaki, Erika
AU - Iriki, Atsushi
AU - Okano, Hideyuki
AU - Mitra, Partha
PY - 2019/2/5
Y1 - 2019/2/5
N2 - Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20 µm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labeled sections. Systematic in-vivo MRI of the individual animals facilitates injection placement into reference-atlas defined anatomical compartments. Further, by combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to accurately map individual brains into a common reference atlas despite the significant individual variation. This approach will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain.
AB - Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20 µm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labeled sections. Systematic in-vivo MRI of the individual animals facilitates injection placement into reference-atlas defined anatomical compartments. Further, by combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to accurately map individual brains into a common reference atlas despite the significant individual variation. This approach will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain.
KW - annotation
KW - high-throughput pipeline
KW - marmoset
KW - mesoscale
KW - neuroscience
KW - registration
KW - tape-transfer method
UR - http://www.scopus.com/inward/record.url?scp=85061957989&partnerID=8YFLogxK
U2 - 10.7554/eLife.40042
DO - 10.7554/eLife.40042
M3 - Article
VL - 8
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e40042
ER -