Projects per year
Abstract
Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20 µm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labeled sections. Systematic in-vivo MRI of the individual animals facilitates injection placement into reference-atlas defined anatomical compartments. Further, by combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to accurately map individual brains into a common reference atlas despite the significant individual variation. This approach will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain.
Original language | English |
---|---|
Article number | e40042 |
Number of pages | 36 |
Journal | eLife |
Volume | 8 |
DOIs | |
Publication status | Published - 5 Feb 2019 |
Keywords
- annotation
- high-throughput pipeline
- marmoset
- mesoscale
- neuroscience
- registration
- tape-transfer method
Projects
- 1 Finished
-
ARC Centre of Excellence for Integrative Brain Function
Egan, G., Rosa, M., Lowery, A., Stuart, G., Arabzadeh, E., Skafidas, E., Ibbotson, M., Petrou, S., Paxinos, G., Mattingley, J., Garrido, M., Sah, P., Robinson, P. A., Martin, P., Grunert, U., Tanaka, K., Mitra, P., Johnson, G., Diamond, M., Margrie, T., Leopold, D., Movshon, J., Markram, H., Victor, J., Hill, S. & Jirsa, V.
Australian National University , ETH Zurich, Australian Research Council (ARC), Karolinska Institute, QIMR Berghofer Medical Research Institute, Ecole Polytechnique Federale de Lausanne (Swiss Federal Institute of Technology in Lausanne) , Monash University, University of Melbourne, University of New South Wales, University of Queensland , University of Sydney, Monash University – Internal University Contribution, National Institutes of Health (United States), Cornell University, New York University, MRC National Institute for Medical Research, Scuola Internazionale Superiore di Studi Avanzati (SISSA), Duke University, Cold Spring Harbor Laboratory, RIKEN
25/06/14 → 31/12/21
Project: Research