A high-throughput in vitro model of human embryo attachment

Huiting Ho, Harmeet Singh, Mohamad Aljofan, Guiying Nie

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells. DESIGN: Experimental study to establish and validate the model. SETTING: Cell culture. CELL(S): Lines BeWo, RL95-2, and AN3-CA. INTERVENTION(S): Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader. MAIN OUTCOME MEASURE(S): Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation. RESULT(S): The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate. CONCLUSION(S): The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment.
Original languageEnglish
Pages (from-to)974 - 978
Number of pages5
JournalFertility and Sterility
Volume97
Issue number4
DOIs
Publication statusPublished - 2012

Cite this

Ho, Huiting ; Singh, Harmeet ; Aljofan, Mohamad ; Nie, Guiying. / A high-throughput in vitro model of human embryo attachment. In: Fertility and Sterility. 2012 ; Vol. 97, No. 4. pp. 974 - 978.
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author = "Huiting Ho and Harmeet Singh and Mohamad Aljofan and Guiying Nie",
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A high-throughput in vitro model of human embryo attachment. / Ho, Huiting; Singh, Harmeet; Aljofan, Mohamad; Nie, Guiying.

In: Fertility and Sterility, Vol. 97, No. 4, 2012, p. 974 - 978.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A high-throughput in vitro model of human embryo attachment

AU - Ho, Huiting

AU - Singh, Harmeet

AU - Aljofan, Mohamad

AU - Nie, Guiying

PY - 2012

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N2 - To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells. DESIGN: Experimental study to establish and validate the model. SETTING: Cell culture. CELL(S): Lines BeWo, RL95-2, and AN3-CA. INTERVENTION(S): Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader. MAIN OUTCOME MEASURE(S): Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation. RESULT(S): The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate. CONCLUSION(S): The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment.

AB - To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells. DESIGN: Experimental study to establish and validate the model. SETTING: Cell culture. CELL(S): Lines BeWo, RL95-2, and AN3-CA. INTERVENTION(S): Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader. MAIN OUTCOME MEASURE(S): Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation. RESULT(S): The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate. CONCLUSION(S): The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment.

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