Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 974 - 978 |
| Number of pages | 5 |
| Journal | Fertility and Sterility |
| Volume | 97 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2012 |
Cite this
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A high-throughput in vitro model of human embryo attachment. / Ho, Huiting; Singh, Harmeet; Aljofan, Mohamad; Nie, Guiying.
In: Fertility and Sterility, Vol. 97, No. 4, 2012, p. 974 - 978.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - A high-throughput in vitro model of human embryo attachment
AU - Ho, Huiting
AU - Singh, Harmeet
AU - Aljofan, Mohamad
AU - Nie, Guiying
PY - 2012
Y1 - 2012
N2 - To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells. DESIGN: Experimental study to establish and validate the model. SETTING: Cell culture. CELL(S): Lines BeWo, RL95-2, and AN3-CA. INTERVENTION(S): Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader. MAIN OUTCOME MEASURE(S): Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation. RESULT(S): The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate. CONCLUSION(S): The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment.
AB - To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells. DESIGN: Experimental study to establish and validate the model. SETTING: Cell culture. CELL(S): Lines BeWo, RL95-2, and AN3-CA. INTERVENTION(S): Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader. MAIN OUTCOME MEASURE(S): Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation. RESULT(S): The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate. CONCLUSION(S): The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment.
UR - http://www.sciencedirect.com/science/article/pii/S0015028212001756
U2 - 10.1016/j.fertnstert.2012.01.116
DO - 10.1016/j.fertnstert.2012.01.116
M3 - Article
VL - 97
SP - 974
EP - 978
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 4
ER -