A high-resolution large area serotonin map of a live rat brain section

Sanjeev K. Kaushalya, Suman Nag, Himanish Ghosh, Senthil Arumugam, Sudipta Maiti

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Abstract

We employ three-photon microscopy to produce a high-resolution map of serotonin autofluorescence in a rat midbrain section (covering more than half of the brain), to quantitatively characterize serotonin distribution and release in different areas of a live brain slice. The map consists of a tiling of ∼160 contiguous optical images (covering an area of ∼27 mm with sub-μm resolution in 20 min), and is recorded before and after inducing depolarization. We observe that the total serotonin exocytosed from the somata in the raphe is quantitatively comparable with regions containing a high density of serotonergic processes. Our results demonstrate that high-resolution, wide-area, dynamic neurotransmitter mapping is now possible.

Original languageEnglish
Pages (from-to)717-721
Number of pages5
JournalNeuroReport
Volume19
Issue number7
DOIs
Publication statusPublished - 1 May 2008
Externally publishedYes

Keywords

  • Macro imaging
  • Neurotransmitter imaging
  • Serotonin
  • Three-photonmicroscopy

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