A generalized transducing phage for the murine pathogen Citrobacter rodentium

Nicola K. Petty, Ana L. Toribio, David Goulding, Ian Foulds, Nicholas Thomson, Gordon Dougan, George P.C. Salmond

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

A virulent phage (φCR1) capable of generalized transduction in Citrobacter rodentium was isolated from the environment and characterized. C. rodentium is a natural pathogen of mice, causing transmissible murine colonic hyperplasia. Sequencing of its genome has recently been completed and will soon be fully annotated and published. C. rodentium is an important model organism for infections caused by the human pathogens enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC). φCR1 uses a lipopolysaccharide receptor, has a genome size of approximately 300 kb, and is able to transduce a variety of markers. φCR1 is the first reported transducing phage for C. rodentium and will be a useful tool for functional genomic analysis of this important natural murine pathogen.

Original languageEnglish
Pages (from-to)2984-2988
Number of pages5
JournalMicrobiology
Volume153
Issue number9
DOIs
Publication statusPublished - 1 Sep 2007
Externally publishedYes

Cite this

Petty, N. K., Toribio, A. L., Goulding, D., Foulds, I., Thomson, N., Dougan, G., & Salmond, G. P. C. (2007). A generalized transducing phage for the murine pathogen Citrobacter rodentium. Microbiology, 153(9), 2984-2988. https://doi.org/10.1099/mic.0.2007/008888-0