@article{543b0d1894d744279b12902f86aea12a,
title = "A General Strategy to Design Highly Fluorogenic Far-Red and Near-Infrared Tetrazine Bioorthogonal Probes",
abstract = "Highly fluorogenic tetrazine bioorthogonal probes emitting at near-infrared wavelengths are in strong demand for biomedical imaging applications. Herein, we have developed a strategy for forming a palette of novel Huaxi-Fluor probes in situ, whose fluorescence increases hundreds of times upon forming the bioorthogonal reaction product, pyridazine. The resulting probes show large Stokes shifts and high quantum yields. Manipulating the conjugate length and pull–push strength in the fluorophore skeleton allows the emission wavelength to be fine-tuned from 556 to 728 nm. The highly photo-stable and biocompatible probes are suitable for visualizing organelles in live cells without a washing step and for imaging of tumors in live small animals to depths of 500 μm by two-photon excitation.",
keywords = "bioorthogonal chemistry, fluorophores, imaging, near-infrared probes, tetrazine",
author = "Wuyu Mao and Jie Tang and Liqun Dai and Xinyu He and Jie Li and Larry Cai and Ping Liao and Ruotian Jiang and Jingwei Zhou and Haoxing Wu",
note = "Funding Information: We acknowledge financial support from National Natural Science Foundation of China (Grant No. 21977075, 21807075). “The Fundamental Research Funds for the Central Universities”. China Postdoctoral Science Foundation (2018M643464). “1.3.5 project for disciplines of excellence, West China Hospital, Sichuan University”. We would like to thank the Analytical & Testing Center of Sichuan University for NMR Tests. Funding Information: We acknowledge financial support from National Natural Science Foundation of China (Grant No. 21977075, 21807075). “The Fundamental Research Funds for the Central Universities”. China Postdoctoral Science Foundation (2018M643464). “1.3.5 project for disciplines of excellence, West China Hospital, Sichuan University”. We would like to thank the Analytical & Testing Center of Sichuan University for NMR Tests. Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH",
year = "2021",
month = feb,
day = "1",
doi = "10.1002/anie.202011544",
language = "English",
volume = "60",
pages = "2393--2397",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "Wiley-Blackwell",
number = "5",
}