A gene regulatory network anchored by LIM homeobox 1 for embryonic head development

Tennille Sibbritt, Chi K. Ip, Poh Lynn Khoo, Emilie Wilkie, Vanessa Jones, Jane Q.J. Sun, Joanne X. Shen, Guangdun Peng, Jing Dong J. Han, Naihe Jing, Pierre Osteil, Mirana Ramialison, Patrick P.L. Tam, Nicolas Fossat

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Development of the embryonic head is driven by the activity of gene regulatory networks of transcription factors. LHX1 is a homeobox transcription factor that plays an essential role in the formation of the embryonic head. The loss of LHX1 function results in anterior truncation of the embryo caused by the disruption of morphogenetic movement of tissue precursors and the dysregulation of WNT signaling activity. Profiling the gene expression pattern in the Lhx1 mutant embryo revealed that tissues in anterior germ layers acquire posterior tissue characteristics, suggesting LHX1 activity is required for the allocation and patterning of head precursor tissues. Here, we used LHX1 as an entry point to delineate its transcriptional targets and interactors and construct a LHX1-anchored gene regulatory network. Using a gain-of-function approach, we identified genes that immediately respond to Lhx1 activation. Meta-analysis of the datasets of LHX1-responsive genes and genes expressed in the anterior tissues of mouse embryos at head-fold stage, in conjunction with published Xenopus embryonic LHX1 (Xlim1) ChIP-seq data, has pinpointed the putative transcriptional targets of LHX1 and an array of genetic determinants functioning together in the formation of the mouse embryonic head.

Original languageEnglish
Article numbere23246
Number of pages13
JournalGenesis
Volume56
Issue number9
DOIs
Publication statusPublished - 1 Sep 2018

Keywords

  • gene regulatory network
  • Lhx1
  • mouse
  • WNT signaling

Cite this

Sibbritt, T., Ip, C. K., Khoo, P. L., Wilkie, E., Jones, V., Sun, J. Q. J., ... Fossat, N. (2018). A gene regulatory network anchored by LIM homeobox 1 for embryonic head development. Genesis, 56(9), [e23246]. https://doi.org/10.1002/dvg.23246
Sibbritt, Tennille ; Ip, Chi K. ; Khoo, Poh Lynn ; Wilkie, Emilie ; Jones, Vanessa ; Sun, Jane Q.J. ; Shen, Joanne X. ; Peng, Guangdun ; Han, Jing Dong J. ; Jing, Naihe ; Osteil, Pierre ; Ramialison, Mirana ; Tam, Patrick P.L. ; Fossat, Nicolas. / A gene regulatory network anchored by LIM homeobox 1 for embryonic head development. In: Genesis. 2018 ; Vol. 56, No. 9.
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abstract = "Development of the embryonic head is driven by the activity of gene regulatory networks of transcription factors. LHX1 is a homeobox transcription factor that plays an essential role in the formation of the embryonic head. The loss of LHX1 function results in anterior truncation of the embryo caused by the disruption of morphogenetic movement of tissue precursors and the dysregulation of WNT signaling activity. Profiling the gene expression pattern in the Lhx1 mutant embryo revealed that tissues in anterior germ layers acquire posterior tissue characteristics, suggesting LHX1 activity is required for the allocation and patterning of head precursor tissues. Here, we used LHX1 as an entry point to delineate its transcriptional targets and interactors and construct a LHX1-anchored gene regulatory network. Using a gain-of-function approach, we identified genes that immediately respond to Lhx1 activation. Meta-analysis of the datasets of LHX1-responsive genes and genes expressed in the anterior tissues of mouse embryos at head-fold stage, in conjunction with published Xenopus embryonic LHX1 (Xlim1) ChIP-seq data, has pinpointed the putative transcriptional targets of LHX1 and an array of genetic determinants functioning together in the formation of the mouse embryonic head.",
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author = "Tennille Sibbritt and Ip, {Chi K.} and Khoo, {Poh Lynn} and Emilie Wilkie and Vanessa Jones and Sun, {Jane Q.J.} and Shen, {Joanne X.} and Guangdun Peng and Han, {Jing Dong J.} and Naihe Jing and Pierre Osteil and Mirana Ramialison and Tam, {Patrick P.L.} and Nicolas Fossat",
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Sibbritt, T, Ip, CK, Khoo, PL, Wilkie, E, Jones, V, Sun, JQJ, Shen, JX, Peng, G, Han, JDJ, Jing, N, Osteil, P, Ramialison, M, Tam, PPL & Fossat, N 2018, 'A gene regulatory network anchored by LIM homeobox 1 for embryonic head development' Genesis, vol. 56, no. 9, e23246. https://doi.org/10.1002/dvg.23246

A gene regulatory network anchored by LIM homeobox 1 for embryonic head development. / Sibbritt, Tennille; Ip, Chi K.; Khoo, Poh Lynn; Wilkie, Emilie; Jones, Vanessa; Sun, Jane Q.J.; Shen, Joanne X.; Peng, Guangdun; Han, Jing Dong J.; Jing, Naihe; Osteil, Pierre; Ramialison, Mirana; Tam, Patrick P.L.; Fossat, Nicolas.

In: Genesis, Vol. 56, No. 9, e23246, 01.09.2018.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - A gene regulatory network anchored by LIM homeobox 1 for embryonic head development

AU - Sibbritt, Tennille

AU - Ip, Chi K.

AU - Khoo, Poh Lynn

AU - Wilkie, Emilie

AU - Jones, Vanessa

AU - Sun, Jane Q.J.

AU - Shen, Joanne X.

AU - Peng, Guangdun

AU - Han, Jing Dong J.

AU - Jing, Naihe

AU - Osteil, Pierre

AU - Ramialison, Mirana

AU - Tam, Patrick P.L.

AU - Fossat, Nicolas

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AB - Development of the embryonic head is driven by the activity of gene regulatory networks of transcription factors. LHX1 is a homeobox transcription factor that plays an essential role in the formation of the embryonic head. The loss of LHX1 function results in anterior truncation of the embryo caused by the disruption of morphogenetic movement of tissue precursors and the dysregulation of WNT signaling activity. Profiling the gene expression pattern in the Lhx1 mutant embryo revealed that tissues in anterior germ layers acquire posterior tissue characteristics, suggesting LHX1 activity is required for the allocation and patterning of head precursor tissues. Here, we used LHX1 as an entry point to delineate its transcriptional targets and interactors and construct a LHX1-anchored gene regulatory network. Using a gain-of-function approach, we identified genes that immediately respond to Lhx1 activation. Meta-analysis of the datasets of LHX1-responsive genes and genes expressed in the anterior tissues of mouse embryos at head-fold stage, in conjunction with published Xenopus embryonic LHX1 (Xlim1) ChIP-seq data, has pinpointed the putative transcriptional targets of LHX1 and an array of genetic determinants functioning together in the formation of the mouse embryonic head.

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KW - WNT signaling

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DO - 10.1002/dvg.23246

M3 - Article

VL - 56

JO - Genesis

JF - Genesis

SN - 1526-954X

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ER -

Sibbritt T, Ip CK, Khoo PL, Wilkie E, Jones V, Sun JQJ et al. A gene regulatory network anchored by LIM homeobox 1 for embryonic head development. Genesis. 2018 Sep 1;56(9). e23246. https://doi.org/10.1002/dvg.23246