A gene expression database for the molecular pharmacology of cancer

Uwe Scherf, Douglas T. Ross, Mark Waltham, Lawrence H. Smith, Jae K. Lee, Lorraine Tanabe, Kurt W. Kohn, William C. Reinhold, Timothy G. Myers, Darren T. Andrews, Dominic A. Scudiero, Michael B. Eisen, Edward A. Sausville, Yves Pommier, David Botstein, Patrick O. Brown, John N. Weinstein

Research output: Contribution to journalArticleResearchpeer-review

1238 Citations (Scopus)

Abstract

We used cDNA microarrays to assess gene expression profiles in 60 human cancer cell lines used in a drug discovery screen by the National Cancer Institute. Using these data, we linked bioinformatics and chemoinformatics by correlating gene expression and drug activity patterns in the NCI60 lines. Clustering the cell lines on the basis of gene expression yielded relationships very different from those obtained by clustering the cell lines on the basis of their response to drugs. Gene-drug relationships for the clinical agents 5-fluorouracil and L-asparaginase exemplify how variations in the transcript levels of particular genes relate to mechanisms of drug sensitivity and resistance. This is the first study to integrate large databases on gene expression and molecular pharmacology.

Original languageEnglish
Pages (from-to)236-244
Number of pages9
JournalNature Genetics
Volume24
Issue number3
DOIs
Publication statusPublished - 1 Mar 2000
Externally publishedYes

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