A Functional Genomics Approach to Henipavirus Research: The Role of Nuclear Proteins, MicroRNAs and Immune Regulators in Infection and Disease

Cameron R. Stewart, Celine Deffrasnes, Chwan Hong Foo, Andrew G.D. Bean, Lin Fa Wang

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Hendra and Nipah viruses (family Paramyxoviridae, genus Henipavirus) are zoonotic RNA viruses that cause lethal disease in humans and are designated as Biosafety Level 4 (BSL4) agents. Moreover, henipaviruses belong to the same group of viruses that cause disease more commonly in humans such as measles, mumps and respiratory syncytial virus. Due to the relatively recent emergence of the henipaviruses and the practical constraints of performing functional genomics studies at high levels of containment, our understanding of the henipavirus infection cycle is incomplete. In this chapter we describe recent loss-of-function (i.e. RNAi) functional genomics screens that shed light on the henipavirus-host interface at a genome-wide level. Further to this, we cross-reference RNAi results with studies probing host proteins targeted by henipavirus proteins, such as nuclear proteins and immune modulators. These functional genomics studies join a growing body of evidence demonstrating that nuclear and nucleolar host proteins play a crucial role in henipavirus infection. Furthermore these studies will underpin future efforts to define the role of nucleolar host-virus interactions in infection and disease.

Original languageEnglish
Title of host publicationRoles of Host Gene and Non-coding RNA Expression in Virus Infection
EditorsRalph A. Tripp, S. Mark Tompkins
PublisherSpringer
Pages191-213
Number of pages23
Volume419
ISBN (Electronic)9783030053697
ISBN (Print)9783030053680
DOIs
Publication statusPublished - 4 Jul 2017

Publication series

NameCurrent topics in microbiology and immunology
ISSN (Print)0070-217X

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