A fully human inhibitory monoclonal antibody to the Wnt receptor RYK

Michael M Halford, Maria L Macheda, Clare Parish, Elena A Takano, Stephen Fox, Daniel Stuart Layton, Edouard Nice, Steven A Stacker

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16 Citations (Scopus)

Abstract

RYK is an unusual member of the receptor tyrosine kinase (RTK) family that is classified as a putative pseudokinase. RYK regulates fundamental biological processes including cell differentiation, migration and target selection, axon outgrowth and pathfinding by transducing signals across the plasma membrane in response to the high affinity binding of Wnt family ligands to its extracellular Wnt inhibitory factor (WIF) domain. Here we report the generation and initial characterization of a fully human inhibitory monoclonal antibody to the human RYK WIF domain. From a naive human single chain fragment variable (scFv) phage display library, we identified anti-RYK WIF domain-specific scFvs then screened for those that could compete with Wnt3a for binding. Production of a fully human IgG1kappa from an inhibitory scFv yielded a monoclonal antibody that inhibits Wnt5a-responsive RYK function in a neurite outgrowth assay. This antibody will have immediate applications for modulating RYK function in a range of settings including development and adult homeostasis, with significant potential for therapeutic use in human pathologies.
Original languageEnglish
Article numbere75447
Number of pages14
JournalPLoS ONE
Volume8
Issue number9
DOIs
Publication statusPublished - 2013

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