A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry

Amandeep Kaur; Mohammad A. Haghighatbin; Conor F. Hogan; Elizabeth J. New

doi:10.1039/c5cc03394b

A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry

Amandeep Kaur, Mohammad A. Haghighatbin, Conor F. Hogan, Elizabeth J. New

Research output: Contribution to journal › Article › Research › peer-review

60 Citations (Scopus)

Abstract

Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.

Original language	English
Pages (from-to)	10510-10513
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	52
DOIs	https://doi.org/10.1039/c5cc03394b
Publication status	Published - 4 Jul 2015
Externally published	Yes

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title = "A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry",

abstract = "Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.",

author = "Amandeep Kaur and Haghighatbin, {Mohammad A.} and Hogan, {Conor F.} and New, {Elizabeth J.}",

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AU - Kaur, Amandeep

AU - Haghighatbin, Mohammad A.

AU - Hogan, Conor F.

AU - New, Elizabeth J.

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2015/7/4

Y1 - 2015/7/4

N2 - Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.

AB - Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities - confocal microscopy, fluorescence lifetime imaging and flow cytometry.

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SP - 10510

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JO - Chemical Communications

JF - Chemical Communications

IS - 52

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A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry

Abstract

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