A fluorogenic probe based on chelation-hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

Gaobin Zhang, Yanfei Zhao, Bo Peng, Zheng Li, Chenchen Xu, Yi Liu, Chengwu Zhang, Nicolas H. Voelcker, Lin Li, Wei Huang

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Developing efficient methods for the real-time detection of Zn2+ levels in biological systems is highly relevant to improving our understanding of the role of Zn2+ in the progression of Parkinson's disease (PD). In this work, a novel Schiff base based Zn2+ fluorescent probe (ZP) was designed, synthesized and systematically investigated. A significant turn-on effect on ZP upon the addition of Zn2+ was observed, accompanied by a blue-shift of the fluorescence spectra. ZP is sensitive to Zn2+ and has excellent selectivity against various biologically relevant cations, anions and amino acids. The sensing mechanism of ZP was studied by 1H NMR, MS, single crystal X-ray diffraction and theoretical calculations. The results showed that the response of ZP to Zn2+ was based on the chelation-hydrolysis-enhancement process. Upon bonding, Zn2+ hydrolyzes the Schiff base to an aldehyde precursor, the resulting aldehyde further coordinates to Zn2+ to form a more stable heterobimetallic complex leading to the emission enhancement and blue-shift. ZP was applied to imaging exogenous/endogenous Zn2+ in live HeLa cells. Furthermore, we successfully measured the Zn2+ levels using in vitro PD models, which provided a visualization method to better understand the relationship between Zn2+ levels and PD development.

Original languageEnglish
Pages (from-to)2252-2260
Number of pages9
JournalJournal of Materials Chemistry B
Volume7
Issue number14
DOIs
Publication statusPublished - 14 Apr 2019

Cite this

Zhang, Gaobin ; Zhao, Yanfei ; Peng, Bo ; Li, Zheng ; Xu, Chenchen ; Liu, Yi ; Zhang, Chengwu ; Voelcker, Nicolas H. ; Li, Lin ; Huang, Wei. / A fluorogenic probe based on chelation-hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models. In: Journal of Materials Chemistry B. 2019 ; Vol. 7, No. 14. pp. 2252-2260.
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abstract = "Developing efficient methods for the real-time detection of Zn2+ levels in biological systems is highly relevant to improving our understanding of the role of Zn2+ in the progression of Parkinson's disease (PD). In this work, a novel Schiff base based Zn2+ fluorescent probe (ZP) was designed, synthesized and systematically investigated. A significant turn-on effect on ZP upon the addition of Zn2+ was observed, accompanied by a blue-shift of the fluorescence spectra. ZP is sensitive to Zn2+ and has excellent selectivity against various biologically relevant cations, anions and amino acids. The sensing mechanism of ZP was studied by 1H NMR, MS, single crystal X-ray diffraction and theoretical calculations. The results showed that the response of ZP to Zn2+ was based on the chelation-hydrolysis-enhancement process. Upon bonding, Zn2+ hydrolyzes the Schiff base to an aldehyde precursor, the resulting aldehyde further coordinates to Zn2+ to form a more stable heterobimetallic complex leading to the emission enhancement and blue-shift. ZP was applied to imaging exogenous/endogenous Zn2+ in live HeLa cells. Furthermore, we successfully measured the Zn2+ levels using in vitro PD models, which provided a visualization method to better understand the relationship between Zn2+ levels and PD development.",
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A fluorogenic probe based on chelation-hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models. / Zhang, Gaobin; Zhao, Yanfei; Peng, Bo; Li, Zheng; Xu, Chenchen; Liu, Yi; Zhang, Chengwu; Voelcker, Nicolas H.; Li, Lin; Huang, Wei.

In: Journal of Materials Chemistry B, Vol. 7, No. 14, 14.04.2019, p. 2252-2260.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Zhang, Gaobin

AU - Zhao, Yanfei

AU - Peng, Bo

AU - Li, Zheng

AU - Xu, Chenchen

AU - Liu, Yi

AU - Zhang, Chengwu

AU - Voelcker, Nicolas H.

AU - Li, Lin

AU - Huang, Wei

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