A fluorescent microsphere-based method for assay of multiple analytes in plasma

Oliver K. Bernhard, Rommel A. Mathias, Thomas W. Barnes, Richard J Simpson

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

9 Citations (Scopus)

Abstract

Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the "gold standard" for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex). 

Original languageEnglish
Title of host publicationSerum/Plasma Proteomics
Subtitle of host publicationMethods and Protocols
EditorsRichard J. Simpson, David W. Greening
Place of PublicationNew York NY USA
PublisherHumana Press
Pages195-206
Number of pages12
Volume728
Edition1
ISBN (Electronic)9781617790683
ISBN (Print)9781617790676
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume728
ISSN (Print)10643745
ISSN (Electronic)1940-6029

Keywords

  • Biomarker validation
  • Flow cytometry
  • Fluorescent beads
  • Multiplex assay
  • Plasma

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