A fluorescent microsphere-based method for assay of multiple analytes in plasma

Oliver K. Bernhard, Rommel A. Mathias, Thomas W. Barnes, Richard J Simpson

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

9 Citations (Scopus)


Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the "gold standard" for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex). 

Original languageEnglish
Title of host publicationSerum/Plasma Proteomics
Subtitle of host publicationMethods and Protocols
EditorsRichard J. Simpson, David W. Greening
Place of PublicationNew York NY USA
PublisherHumana Press
Number of pages12
ISBN (Electronic)9781617790683
ISBN (Print)9781617790676
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)10643745
ISSN (Electronic)1940-6029


  • Biomarker validation
  • Flow cytometry
  • Fluorescent beads
  • Multiplex assay
  • Plasma

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