A fluorescent microsphere-based method for assay of multiple analytes in plasma

Oliver K. Bernhard; Rommel A. Mathias; Thomas W. Barnes; Richard J Simpson

doi:10.1007/978-1-61779-068-3_12

A fluorescent microsphere-based method for assay of multiple analytes in plasma

Oliver K. Bernhard, Rommel A. Mathias, Thomas W. Barnes, Richard J Simpson

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

11 Citations (Scopus)

Abstract

Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the "gold standard" for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex).

Original language	English
Title of host publication	Serum/Plasma Proteomics
Subtitle of host publication	Methods and Protocols
Editors	Richard J. Simpson, David W. Greening
Place of Publication	New York NY USA
Publisher	Humana Press
Pages	195-206
Number of pages	12
Volume	728
Edition	1
ISBN (Electronic)	9781617790683
ISBN (Print)	9781617790676
DOIs	https://doi.org/10.1007/978-1-61779-068-3_12
Publication status	Published - 2011
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	728
ISSN (Print)	10643745
ISSN (Electronic)	1940-6029

Keywords

Biomarker validation
Flow cytometry
Fluorescent beads
Multiplex assay
Plasma

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-61779-068-3_12

Cite this

Bernhard, O. K., Mathias, R. A., Barnes, T. W., & Simpson, R. J. (2011). A fluorescent microsphere-based method for assay of multiple analytes in plasma. In R. J. Simpson, & D. W. Greening (Eds.), Serum/Plasma Proteomics: Methods and Protocols (1 ed., Vol. 728, pp. 195-206). (Methods in Molecular Biology; Vol. 728). Humana Press. https://doi.org/10.1007/978-1-61779-068-3_12

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keywords = "Biomarker validation, Flow cytometry, Fluorescent beads, Multiplex assay, Plasma",

author = "Bernhard, {Oliver K.} and Mathias, {Rommel A.} and Barnes, {Thomas W.} and Simpson, {Richard J}",

year = "2011",

doi = "10.1007/978-1-61779-068-3_12",

language = "English",

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volume = "728",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

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A fluorescent microsphere-based method for assay of multiple analytes in plasma. / Bernhard, Oliver K.; Mathias, Rommel A.; Barnes, Thomas W. et al.
Serum/Plasma Proteomics: Methods and Protocols. ed. / Richard J. Simpson; David W. Greening. Vol. 728 1. ed. New York NY USA: Humana Press, 2011. p. 195-206 (Methods in Molecular Biology; Vol. 728).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

TY - CHAP

T1 - A fluorescent microsphere-based method for assay of multiple analytes in plasma

AU - Bernhard, Oliver K.

AU - Mathias, Rommel A.

AU - Barnes, Thomas W.

AU - Simpson, Richard J

PY - 2011

Y1 - 2011

N2 - Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the "gold standard" for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex).

AB - Measurement of multiple analytes can provide increased sensitivity and specificity for the detection and management of disease. The enzyme-linked immunosorbent assay (ELISA) is currently the "gold standard" for protein quantification; however, individual assays for each analyte must be performed, placing demand on sample volume. On the contrary, multiplex assays using microsphere-based technologies allow for multiple analytes to be simultaneously assayed within a single sample. Here, we present a protocol for the preparation and development of a multiple-analyte assay in human plasma using the BioPlex 200 platform (Bio-Rad), which incorporates xMAP technology (Luminex).

KW - Biomarker validation

KW - Flow cytometry

KW - Fluorescent beads

KW - Multiplex assay

KW - Plasma

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AN - SCOPUS:79960128592

SN - 9781617790676

VL - 728

T3 - Methods in Molecular Biology

SP - 195

EP - 206

BT - Serum/Plasma Proteomics

A2 - Simpson, Richard J.

A2 - Greening, David W.

PB - Humana Press

CY - New York NY USA

ER -

A fluorescent microsphere-based method for assay of multiple analytes in plasma

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Colorectal cancer - molecular basis to targeted therapeutics

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A fluorescent microsphere-based method for assay of multiple analytes in plasma

Abstract

Publication series

Keywords

UN SDGs

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Colorectal cancer - molecular basis to targeted therapeutics

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