A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging

Cassandra L. Fleming; Trent Ashton; Cameron Nowell; Mark Devlin; Anthony Natoli; Jeannette Schreuders; Frederick M. Pfeffer

doi:10.1039/c5cc02059j

A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging

Cassandra L. Fleming, Trent Ashton, Cameron Nowell, Mark Devlin, Anthony Natoli, Jeannette Schreuders, Frederick M. Pfeffer

Drug Discovery Biology

Research output: Contribution to journal › Letter › Research › peer-review

34 Citations (Scopus)

Abstract

Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify 'profluorogenic' substrates for intracellular studies.

Original language	English
Pages (from-to)	7827-7830
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	37
DOIs	https://doi.org/10.1039/c5cc02059j
Publication status	Published - 7 May 2015

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abstract = "Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify 'profluorogenic' substrates for intracellular studies.",

author = "Fleming, {Cassandra L.} and Trent Ashton and Cameron Nowell and Mark Devlin and Anthony Natoli and Jeannette Schreuders and Pfeffer, {Frederick M.}",

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T1 - A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging

AU - Fleming, Cassandra L.

AU - Ashton, Trent

AU - Nowell, Cameron

AU - Devlin, Mark

AU - Natoli, Anthony

AU - Schreuders, Jeannette

AU - Pfeffer, Frederick M.

PY - 2015/5/7

Y1 - 2015/5/7

N2 - Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify 'profluorogenic' substrates for intracellular studies.

AB - Fluorescence microscopy studies using 4-morpholinoscriptaid (4MS) demonstrated rapid cellular uptake of this scriptaid analogue into the cytoplasm but no nuclear penetration. As 4MS and scriptaid have the same in vitro activity against HDACs and KASUMI-1 cells; 4MS exemplifies a rational approach to subtly modify 'profluorogenic' substrates for intracellular studies.

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M3 - Letter

C2 - 25853994

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SN - 1359-7345

VL - 51

SP - 7827

EP - 7830

JO - Chemical Communications

JF - Chemical Communications

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ER -

A fluorescent histone deacetylase (HDAC) inhibitor for cellular imaging

Abstract

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