A dynamic analysis of muscle fusion in the chick embryo

Daniel Sieiro-Mosti, Marie De La Celle, Manuel Pele, Christophe Marcelle

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Skeletal muscle development, growth and regeneration depend upon the ability of muscle cells to fuse into multinucleated fibers. Surprisingly little is known about the cellular events that underlie fusion during amniote development. Here, we have developed novel molecular tools to characterize muscle cell fusion during chick embryo development. We show that all cell populations arising from somites fuse, but each with unique characteristics. Fusion in the trunk is slow and independent of fiber length. By contrast, the addition of nuclei in limb muscles is three times more rapid than in trunk and is tightly associated with fiber growth. A complex interaction takes place in the trunk, where primary myotome cells from the medial somite border rarely fuse to one another, but readily do so with anterior and posterior border cells. Conversely, resident muscle progenitors actively fuse with one another, but poorly with the primary myotome. In summary, this study unveils an unexpected variety of fusion behaviors in distinct embryonic domains that is likely to reflect a tight molecular control of muscle fusion in vertebrates.
Original languageEnglish
Pages (from-to)3605 - 3611
Number of pages7
JournalDevelopment
Volume141
Issue number18
DOIs
Publication statusPublished - 2014

Cite this

Sieiro-Mosti, Daniel ; De La Celle, Marie ; Pele, Manuel ; Marcelle, Christophe. / A dynamic analysis of muscle fusion in the chick embryo. In: Development. 2014 ; Vol. 141, No. 18. pp. 3605 - 3611.
@article{dd55abe4ad224d77aef6b0a76f156dad,
title = "A dynamic analysis of muscle fusion in the chick embryo",
abstract = "Skeletal muscle development, growth and regeneration depend upon the ability of muscle cells to fuse into multinucleated fibers. Surprisingly little is known about the cellular events that underlie fusion during amniote development. Here, we have developed novel molecular tools to characterize muscle cell fusion during chick embryo development. We show that all cell populations arising from somites fuse, but each with unique characteristics. Fusion in the trunk is slow and independent of fiber length. By contrast, the addition of nuclei in limb muscles is three times more rapid than in trunk and is tightly associated with fiber growth. A complex interaction takes place in the trunk, where primary myotome cells from the medial somite border rarely fuse to one another, but readily do so with anterior and posterior border cells. Conversely, resident muscle progenitors actively fuse with one another, but poorly with the primary myotome. In summary, this study unveils an unexpected variety of fusion behaviors in distinct embryonic domains that is likely to reflect a tight molecular control of muscle fusion in vertebrates.",
author = "Daniel Sieiro-Mosti and {De La Celle}, Marie and Manuel Pele and Christophe Marcelle",
year = "2014",
doi = "10.1242/dev.114546",
language = "English",
volume = "141",
pages = "3605 -- 3611",
journal = "Development",
issn = "0950-1991",
publisher = "The Company of Biologists Ltd",
number = "18",

}

A dynamic analysis of muscle fusion in the chick embryo. / Sieiro-Mosti, Daniel; De La Celle, Marie; Pele, Manuel; Marcelle, Christophe.

In: Development, Vol. 141, No. 18, 2014, p. 3605 - 3611.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A dynamic analysis of muscle fusion in the chick embryo

AU - Sieiro-Mosti, Daniel

AU - De La Celle, Marie

AU - Pele, Manuel

AU - Marcelle, Christophe

PY - 2014

Y1 - 2014

N2 - Skeletal muscle development, growth and regeneration depend upon the ability of muscle cells to fuse into multinucleated fibers. Surprisingly little is known about the cellular events that underlie fusion during amniote development. Here, we have developed novel molecular tools to characterize muscle cell fusion during chick embryo development. We show that all cell populations arising from somites fuse, but each with unique characteristics. Fusion in the trunk is slow and independent of fiber length. By contrast, the addition of nuclei in limb muscles is three times more rapid than in trunk and is tightly associated with fiber growth. A complex interaction takes place in the trunk, where primary myotome cells from the medial somite border rarely fuse to one another, but readily do so with anterior and posterior border cells. Conversely, resident muscle progenitors actively fuse with one another, but poorly with the primary myotome. In summary, this study unveils an unexpected variety of fusion behaviors in distinct embryonic domains that is likely to reflect a tight molecular control of muscle fusion in vertebrates.

AB - Skeletal muscle development, growth and regeneration depend upon the ability of muscle cells to fuse into multinucleated fibers. Surprisingly little is known about the cellular events that underlie fusion during amniote development. Here, we have developed novel molecular tools to characterize muscle cell fusion during chick embryo development. We show that all cell populations arising from somites fuse, but each with unique characteristics. Fusion in the trunk is slow and independent of fiber length. By contrast, the addition of nuclei in limb muscles is three times more rapid than in trunk and is tightly associated with fiber growth. A complex interaction takes place in the trunk, where primary myotome cells from the medial somite border rarely fuse to one another, but readily do so with anterior and posterior border cells. Conversely, resident muscle progenitors actively fuse with one another, but poorly with the primary myotome. In summary, this study unveils an unexpected variety of fusion behaviors in distinct embryonic domains that is likely to reflect a tight molecular control of muscle fusion in vertebrates.

UR - http://dev.biologists.org/content/141/18/3605.full.pdf

U2 - 10.1242/dev.114546

DO - 10.1242/dev.114546

M3 - Article

VL - 141

SP - 3605

EP - 3611

JO - Development

JF - Development

SN - 0950-1991

IS - 18

ER -