TY - JOUR
T1 - A doubly amplified electrochemical immunoassay for carcinoembryonic antigen
AU - Gao, Zhiqiang
AU - Zhang, Jie
AU - Ting, Boon Ping
PY - 2009
Y1 - 2009
N2 - An ultrasensitive electrochemical immunoassay(EIA) for the detection of carcinoembryonic antigen (CEA) is described in this report. The assay involves utilizing enzyme-catalyzed deposition of a redox polymer and electrocatalytic oxidation of ascorbic acid (AA) by the deposited redox polymer, a dual-amplification scheme to enhance analytical signals. Briefly, CEA capturing antibody and redox polymer anchoring agent were covalently immobilized on a gold electrode. After incubating with CEA, the electrode was treated in detection antibody-glucose oxidase conjugate solution. Thereafter, it was dipped into the redox polymer solution. Upon the addition of glucose, the redox polymer was enzymatically reduced and deposited on the electrode surface. The deposited redox polymer exhibits excellent electrocatalytic activity towards the oxidation of AA. Consequently, CEA could be quantified amperometrically. This electrochemical immunoassay combines the specificity of the immunological reaction with the sensitivity of the doubly amplified electrochemical detection. (C) 2008 Elsevier B.V. All rights reserved
AB - An ultrasensitive electrochemical immunoassay(EIA) for the detection of carcinoembryonic antigen (CEA) is described in this report. The assay involves utilizing enzyme-catalyzed deposition of a redox polymer and electrocatalytic oxidation of ascorbic acid (AA) by the deposited redox polymer, a dual-amplification scheme to enhance analytical signals. Briefly, CEA capturing antibody and redox polymer anchoring agent were covalently immobilized on a gold electrode. After incubating with CEA, the electrode was treated in detection antibody-glucose oxidase conjugate solution. Thereafter, it was dipped into the redox polymer solution. Upon the addition of glucose, the redox polymer was enzymatically reduced and deposited on the electrode surface. The deposited redox polymer exhibits excellent electrocatalytic activity towards the oxidation of AA. Consequently, CEA could be quantified amperometrically. This electrochemical immunoassay combines the specificity of the immunological reaction with the sensitivity of the doubly amplified electrochemical detection. (C) 2008 Elsevier B.V. All rights reserved
UR - http://www.sciencedirect.com.ezproxy.lib.monash.edu.au/science?_ob=MImg&_imagekey=B6TFC-4TGS7CM-4-F&_cdi=5223&_user=542840&_pii=S0956566308005319&_ori
UR - https://www.scopus.com/pages/publications/60349121699
U2 - 10.1016/j.bios.2008.09.009
DO - 10.1016/j.bios.2008.09.009
M3 - Article
SN - 0956-5663
VL - 24
SP - 1825
EP - 1830
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -