A dominant Vβ bias in the CTL response after HSV-1 infection is determined by peptide residues predicted to also interact with the TCR β- chain CDR3

Stephen J. Turner, Francis R. Carbone

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Many T cell responses are dominated by restricted TCR expression and can range from repeated usage of particular TCR Vβ-and/or Vα-elements, to the preferential usage of both V- and J-elements, often in conjunction with conserved V-D-J or V-J junctional sequences. Cytotoxic T lymphocytes specific for a K(b)-restricted determinant from the herpes simplex virus glycoprotein B (gB) preferentially express a dominant TCRBV10 β-chain with sequence conservation of a tryptophan-glycine located in the V-D junction. Here we have examined whether immunisation of C57BL/6 mice with the gB-peptide can mimic the CTL response seen after HSV1 infection. Immunisation with the gB- peptide resulted in the generation of gB-specific CTL that showed a similar TCRBV10 bias to that observed after HSV-1 infection. When the gB-determinant was expressed as a part of a fusion protein, immunised mice again exhibited the TCRBV10 bias with the junctional sequence conservation in the responding CTL. C57BL/6 mice were then immunised with variants of the gB-peptide that contained amino acid substitutions at positions previously predicted to contact the TCR β-chain CDR3. Analysis of the TCRBV usage of variant specific CTL lines showed that substitutions at the TCR-contact positions 4, 6 and 7 of the gB-peptide resulted in a loss of the TCRBV10 bias. These results suggest that the TCRBV10 bias seen in gB-specific CTL after HSV-1 infection is due to antigenic selection by the minimal peptide and is determined by residues proposed to contact the TCR β-chain CDR3.

Original languageEnglish
Pages (from-to)307-316
Number of pages10
JournalMolecular Immunology
Volume35
Issue number5
DOIs
Publication statusPublished - 1 Apr 1998
Externally publishedYes

Keywords

  • Glycoprotein B
  • Herpes simplex virus
  • T cell receptor
  • V-region bias

Cite this

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title = "A dominant Vβ bias in the CTL response after HSV-1 infection is determined by peptide residues predicted to also interact with the TCR β- chain CDR3",
abstract = "Many T cell responses are dominated by restricted TCR expression and can range from repeated usage of particular TCR Vβ-and/or Vα-elements, to the preferential usage of both V- and J-elements, often in conjunction with conserved V-D-J or V-J junctional sequences. Cytotoxic T lymphocytes specific for a K(b)-restricted determinant from the herpes simplex virus glycoprotein B (gB) preferentially express a dominant TCRBV10 β-chain with sequence conservation of a tryptophan-glycine located in the V-D junction. Here we have examined whether immunisation of C57BL/6 mice with the gB-peptide can mimic the CTL response seen after HSV1 infection. Immunisation with the gB- peptide resulted in the generation of gB-specific CTL that showed a similar TCRBV10 bias to that observed after HSV-1 infection. When the gB-determinant was expressed as a part of a fusion protein, immunised mice again exhibited the TCRBV10 bias with the junctional sequence conservation in the responding CTL. C57BL/6 mice were then immunised with variants of the gB-peptide that contained amino acid substitutions at positions previously predicted to contact the TCR β-chain CDR3. Analysis of the TCRBV usage of variant specific CTL lines showed that substitutions at the TCR-contact positions 4, 6 and 7 of the gB-peptide resulted in a loss of the TCRBV10 bias. These results suggest that the TCRBV10 bias seen in gB-specific CTL after HSV-1 infection is due to antigenic selection by the minimal peptide and is determined by residues proposed to contact the TCR β-chain CDR3.",
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A dominant Vβ bias in the CTL response after HSV-1 infection is determined by peptide residues predicted to also interact with the TCR β- chain CDR3. / Turner, Stephen J.; Carbone, Francis R.

In: Molecular Immunology, Vol. 35, No. 5, 01.04.1998, p. 307-316.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - A dominant Vβ bias in the CTL response after HSV-1 infection is determined by peptide residues predicted to also interact with the TCR β- chain CDR3

AU - Turner, Stephen J.

AU - Carbone, Francis R.

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N2 - Many T cell responses are dominated by restricted TCR expression and can range from repeated usage of particular TCR Vβ-and/or Vα-elements, to the preferential usage of both V- and J-elements, often in conjunction with conserved V-D-J or V-J junctional sequences. Cytotoxic T lymphocytes specific for a K(b)-restricted determinant from the herpes simplex virus glycoprotein B (gB) preferentially express a dominant TCRBV10 β-chain with sequence conservation of a tryptophan-glycine located in the V-D junction. Here we have examined whether immunisation of C57BL/6 mice with the gB-peptide can mimic the CTL response seen after HSV1 infection. Immunisation with the gB- peptide resulted in the generation of gB-specific CTL that showed a similar TCRBV10 bias to that observed after HSV-1 infection. When the gB-determinant was expressed as a part of a fusion protein, immunised mice again exhibited the TCRBV10 bias with the junctional sequence conservation in the responding CTL. C57BL/6 mice were then immunised with variants of the gB-peptide that contained amino acid substitutions at positions previously predicted to contact the TCR β-chain CDR3. Analysis of the TCRBV usage of variant specific CTL lines showed that substitutions at the TCR-contact positions 4, 6 and 7 of the gB-peptide resulted in a loss of the TCRBV10 bias. These results suggest that the TCRBV10 bias seen in gB-specific CTL after HSV-1 infection is due to antigenic selection by the minimal peptide and is determined by residues proposed to contact the TCR β-chain CDR3.

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KW - Glycoprotein B

KW - Herpes simplex virus

KW - T cell receptor

KW - V-region bias

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JF - Molecular Immunology

SN - 0161-5890

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