A DNA biosensor based on the detection of doxorubicin-conjugated Ag nanoparticle labels using solid-state voltammetry

Boon Ping Ting, Jie Zhang, Zhiqiang Gao, Jackie Y Ying

Research output: Contribution to journalArticleResearchpeer-review

63 Citations (Scopus)

Abstract

This report describes an electrochemical biosensor for the detection of short DNA oligonucleotide of the avian flu virus H5N1 with sequence 5a??-CCA AGC AAC AGA CTC AAA-3a??. To fabricate this DNA biosensor, a gold (Au) electrode surface was modified with thiolated DNA probes with a sequence complementary to the target DNA. This modified Au electrode was incubated in a buffer solution containing the target DNA to form double-stranded DNA (ds-DNA) through hybridization. The ds-DNA on the electrode surface was then labeled with silver nanoparticles conjugated with a well-known DNA intercalator, doxorubicin. By performing cyclic voltammetry in an aqueous KCl solution (0.3 M), the silver nanoparticle labels were detected as a result of the highly characteristic solid-state Ag/AgCl redox process. The signal obtained was subsequently used to quantify the amount of DNA. A detection limit of 1 pM has been achieved with this new DNA biosensor.
Original languageEnglish
Pages (from-to)282 - 287
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
DOIs
Publication statusPublished - 2009
Externally publishedYes

Cite this

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abstract = "This report describes an electrochemical biosensor for the detection of short DNA oligonucleotide of the avian flu virus H5N1 with sequence 5a??-CCA AGC AAC AGA CTC AAA-3a??. To fabricate this DNA biosensor, a gold (Au) electrode surface was modified with thiolated DNA probes with a sequence complementary to the target DNA. This modified Au electrode was incubated in a buffer solution containing the target DNA to form double-stranded DNA (ds-DNA) through hybridization. The ds-DNA on the electrode surface was then labeled with silver nanoparticles conjugated with a well-known DNA intercalator, doxorubicin. By performing cyclic voltammetry in an aqueous KCl solution (0.3 M), the silver nanoparticle labels were detected as a result of the highly characteristic solid-state Ag/AgCl redox process. The signal obtained was subsequently used to quantify the amount of DNA. A detection limit of 1 pM has been achieved with this new DNA biosensor.",
author = "Ting, {Boon Ping} and Jie Zhang and Zhiqiang Gao and Ying, {Jackie Y}",
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A DNA biosensor based on the detection of doxorubicin-conjugated Ag nanoparticle labels using solid-state voltammetry. / Ting, Boon Ping; Zhang, Jie; Gao, Zhiqiang; Ying, Jackie Y.

In: Biosensors and Bioelectronics, Vol. 25, 2009, p. 282 - 287.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A DNA biosensor based on the detection of doxorubicin-conjugated Ag nanoparticle labels using solid-state voltammetry

AU - Ting, Boon Ping

AU - Zhang, Jie

AU - Gao, Zhiqiang

AU - Ying, Jackie Y

PY - 2009

Y1 - 2009

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