TY - JOUR
T1 - A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei
AU - Jayaraj, Rama
AU - Hales, Belinda
AU - Viberg, Linda
AU - Pizzuto, Susan
AU - Holt, Deborah
AU - Rolland, Jennifer May
AU - O'Hehir, Robyn E
AU - Currie, Bart J
AU - Walton, Shelley
PY - 2011
Y1 - 2011
N2 - Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50 accuracy. To develop more reliable
diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma
samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA).
Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar
s 14.3 DELFIA showed excellent diagnostic capability, with 100 sensitivity and 93.75 specificity for distinguishing subjects with current
scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s
14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies
infestation in clinical practice.
AB - Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50 accuracy. To develop more reliable
diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma
samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA).
Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar
s 14.3 DELFIA showed excellent diagnostic capability, with 100 sensitivity and 93.75 specificity for distinguishing subjects with current
scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s
14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies
infestation in clinical practice.
U2 - 10.1016/j.diagmicrobio.2011.09.007
DO - 10.1016/j.diagmicrobio.2011.09.007
M3 - Article
SN - 0732-8893
VL - 71
SP - 403
EP - 407
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -