Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50 accuracy. To develop more reliable diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA). Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar s 14.3 DELFIA showed excellent diagnostic capability, with 100 sensitivity and 93.75 specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s 14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice.
|Pages (from-to)||403 - 407|
|Number of pages||5|
|Journal||Diagnostic Microbiology and Infectious Disease|
|Publication status||Published - 2011|
Jayaraj, R., Hales, B., Viberg, L., Pizzuto, S., Holt, D., Rolland, J. M., O'Hehir, R. E., Currie, B. J., & Walton, S. (2011). A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei. Diagnostic Microbiology and Infectious Disease, 71(4), 403 - 407. https://doi.org/10.1016/j.diagmicrobio.2011.09.007