A detailed study on understanding glycopolymer library and Con A interactions

Yanzi Gou, Jin Geng, Sarah Jane Richards, James Burns, C. Remzi Becer, D. M. Haddleton

Research output: Contribution to journalArticleResearchpeer-review

54 Citations (Scopus)

Abstract

Synthetic glycopolymers are important natural oligosaccharides mimics for many biological applications. To develop glycopolymeric drugs and therapeutic agents, factors that control the receptor-ligand interaction need to be investigated. A library of well-defined glycopolymers has been prepared by the combination of copper mediated living radical polymerization and CuAAC click reaction via post-functionalization of alkyne-containing precursor polymers with different sugar azides. Employing Concanavalin A as the model receptor, we explored the influence of the nature and densities of different sugars residues (mannose, galactose, and glucose) on the stoichiometry of the cluster, the rate of the cluster formation, the inhibitory potency of the glycopolymers, and the stability of the turbidity through quantitative precipitation assays, turbidimetry assays, inhibitory potency assays, and reversal aggregation assays. The diversities of binding properties contributed by different clustering parameters will make it possible to define the structures of the multivalent ligands and densities of binding epitopes tailor-made for specific functions in the lectin-ligand interaction.

Original languageEnglish
Pages (from-to)2588-2597
Number of pages10
JournalJournal of Polymer Science, Part A: Polymer Chemistry
Volume51
Issue number12
DOIs
Publication statusPublished - 15 Jun 2013
Externally publishedYes

Keywords

  • biomaterials
  • radical polymerization
  • synthesis

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