A cytochrome P450 conserved in insects is involved in cuticle formation

Tamar E Sztal; Henry Chung; Silke Berger; Peter D Currie; Philip Batterham; Phillip J Daborn

doi:10.1371/journal.pone.0036544

A cytochrome P450 conserved in insects is involved in cuticle formation

Tamar E Sztal, Henry Chung, Silke Berger, Peter D Currie, Philip Batterham, Phillip J Daborn

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Abstract

The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.

Original language	English
Article number	e36544
Number of pages	9
Journal	PLoS ONE
Volume	7
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0036544
Publication status	Published - 2012

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title = "A cytochrome P450 conserved in insects is involved in cuticle formation",

abstract = "The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.",

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AU - Sztal, Tamar E

AU - Chung, Henry

AU - Berger, Silke

AU - Currie, Peter D

AU - Batterham, Philip

AU - Daborn, Phillip J

PY - 2012

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AB - The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.

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A cytochrome P450 conserved in insects is involved in cuticle formation

Abstract

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