A cytochrome P450 conserved in insects is involved in cuticle formation

Tamar E Sztal, Henry Chung, Silke Berger, Peter D Currie, Philip Batterham, Phillip J Daborn

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Abstract

The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.
Original languageEnglish
Article numbere36544
Number of pages9
JournalPLoS ONE
Volume7
Issue number5
DOIs
Publication statusPublished - 2012

Cite this

Sztal, Tamar E ; Chung, Henry ; Berger, Silke ; Currie, Peter D ; Batterham, Philip ; Daborn, Phillip J. / A cytochrome P450 conserved in insects is involved in cuticle formation. In: PLoS ONE. 2012 ; Vol. 7, No. 5.
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A cytochrome P450 conserved in insects is involved in cuticle formation. / Sztal, Tamar E; Chung, Henry; Berger, Silke; Currie, Peter D; Batterham, Philip; Daborn, Phillip J.

In: PLoS ONE, Vol. 7, No. 5, e36544, 2012.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Sztal, Tamar E

AU - Chung, Henry

AU - Berger, Silke

AU - Currie, Peter D

AU - Batterham, Philip

AU - Daborn, Phillip J

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AB - The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.

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