Projects per year
Abstract
Multiple protocols have been published for generation of iMGLs from hESCs/iPSCs. To date, there are no guides to assist researchers to determine the most appropriate methodology for microglial studies. To establish a framework to facilitate future microglial studies, we first performed a comparative transcriptional analysis between iMGLs derived using three published datasets, which allowed us to establish the baseline protocol that is most representative of bona fide human microglia. Secondly, using CRISPR to tag the classic microglial marker CX3CR1 with nanoluciferase and tdTomato, we generated and functionally validated a reporter ESC line. Finally, using this cell line, we demonstrated that co-culture of iMGL precursors with human glia and neurons enhanced transcriptional resemblance of iMGLs to ex vivo microglia. Together, our comprehensive molecular analysis and reporter cell line are a useful resource for neurobiologists seeking to use iMGLs for disease modeling and drug screening studies.
Original language | English |
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Pages (from-to) | 1018-1032 |
Number of pages | 15 |
Journal | Stem Cell Reports |
Volume | 14 |
Issue number | 6 |
DOIs | |
Publication status | Published - 9 Jun 2020 |
Keywords
- in-vitro-derived microglia
- integrative molecular analysis
- microglia
- microglia genetic reporter
- PSC differentiation
- RNA-seq
- synapse internalization
Projects
- 2 Finished
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Anti-inflammatory copper complexes for treatment of Alzheimer's Disease
Grubman, A. (Primary Chief Investigator (PCI))
1/08/16 → 5/12/20
Project: Research
Equipment
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Bioinformatics Platform
Deveson, D. (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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FlowCore
Fryga, A. (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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MHTP Medical Genomics Facility
Wilson, T. (Manager)
Hudson Institute - Department of Molecular and Translational ScienceFacility/equipment: Facility