A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvlmJ

Shih Huey E. Tang, Francisco J. Silva, Walter M.K. Tsark, Jeffrey R. Mann

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118 Citations (Scopus)

Abstract

A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvlmJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this "Cre-deleter" transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
Journalgenesis: The Journal of Genetics and Development
Volume32
Issue number3
DOIs
Publication statusPublished - 9 Apr 2002
Externally publishedYes

Keywords

  • Cre recombinase
  • Deleter
  • Gene targeting
  • Hprt
  • Transgene

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