A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvlmJ

Shih Huey E. Tang, Francisco J. Silva, Walter M.K. Tsark, Jeffrey R. Mann

Research output: Contribution to journalArticleResearchpeer-review

112 Citations (Scopus)

Abstract

A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvlmJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this "Cre-deleter" transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalGenesis
Volume32
Issue number3
DOIs
Publication statusPublished - 9 Apr 2002
Externally publishedYes

Keywords

  • Cre recombinase
  • Deleter
  • Gene targeting
  • Hprt
  • Transgene

Cite this

Tang, Shih Huey E. ; Silva, Francisco J. ; Tsark, Walter M.K. ; Mann, Jeffrey R. / A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvlmJ. In: Genesis. 2002 ; Vol. 32, No. 3. pp. 199-202.
@article{f64871fd08f5497eb09fbdb2a7b18bdf,
title = "A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvlmJ",
abstract = "A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvlmJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this {"}Cre-deleter{"} transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene.",
keywords = "Cre recombinase, Deleter, Gene targeting, Hprt, Transgene",
author = "Tang, {Shih Huey E.} and Silva, {Francisco J.} and Tsark, {Walter M.K.} and Mann, {Jeffrey R.}",
year = "2002",
month = "4",
day = "9",
doi = "10.1002/gene.10030",
language = "English",
volume = "32",
pages = "199--202",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Blackwell",
number = "3",

}

A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvlmJ. / Tang, Shih Huey E.; Silva, Francisco J.; Tsark, Walter M.K.; Mann, Jeffrey R.

In: Genesis, Vol. 32, No. 3, 09.04.2002, p. 199-202.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvlmJ

AU - Tang, Shih Huey E.

AU - Silva, Francisco J.

AU - Tsark, Walter M.K.

AU - Mann, Jeffrey R.

PY - 2002/4/9

Y1 - 2002/4/9

N2 - A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvlmJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this "Cre-deleter" transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene.

AB - A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvlmJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this "Cre-deleter" transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene.

KW - Cre recombinase

KW - Deleter

KW - Gene targeting

KW - Hprt

KW - Transgene

UR - http://www.scopus.com/inward/record.url?scp=0036202934&partnerID=8YFLogxK

U2 - 10.1002/gene.10030

DO - 10.1002/gene.10030

M3 - Article

VL - 32

SP - 199

EP - 202

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 3

ER -