The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 22.214.171.124) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.
|Number of pages||4|
|Journal||Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology|
|Publication status||Published - 7 Sep 1982|
- Butyryl-CoA dehydrogenase
- CoA removal
- Ligand removal
- Thiopropyl-Sepharose 6B