A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase

Gary Williamson; Paul C. Engel

doi:10.1016/0167-4838(82)90493-9

A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase

Gary Williamson, Paul C. Engel

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 1.3.99.2) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.

Original language	English
Pages (from-to)	245-248
Number of pages	4
Journal	Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology
Volume	706
Issue number	2
DOIs	https://doi.org/10.1016/0167-4838(82)90493-9
Publication status	Published - 7 Sept 1982
Externally published	Yes

Keywords

Butyryl-CoA dehydrogenase
CoA removal
Ligand removal
Thiopropyl-Sepharose 6B

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10.1016/0167-4838(82)90493-9

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abstract = "The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 1.3.99.2) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.",

keywords = "Butyryl-CoA dehydrogenase, CoA removal, Ligand removal, Thiopropyl-Sepharose 6B",

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AU - Williamson, Gary

AU - Engel, Paul C.

PY - 1982/9/7

Y1 - 1982/9/7

N2 - The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 1.3.99.2) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.

AB - The commercially available gel, 2-pyridyl disulphide hydroxypropyl ether-Sepharose (thiopropyl-Sepharose 6B), can be used to remove bound ligand completely from butyryl-CoA dehydrogenase (EC 1.3.99.2) in two simple operations. The resultant enzyme forms normal complexes with acetoacetyl-CoA and CoA persulphide, contains no bound CoA as determined by the enzymatic assay for CoA, and retains full catalytic activity.

KW - Butyryl-CoA dehydrogenase

KW - CoA removal

KW - Ligand removal

KW - Thiopropyl-Sepharose 6B

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DO - 10.1016/0167-4838(82)90493-9

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AN - SCOPUS:0020476931

SN - 0167-4838

VL - 706

SP - 245

EP - 248

JO - Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

JF - Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

IS - 2

ER -

A convenient and rapid method for the complete removal of CoA from butyryl-CoA dehydrogenase

Abstract

Keywords

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