A computer program for determining the size of DNA restriction fragments

Ronald G. Duggleby; Howard Kinns; Julian I. Rood

doi:10.1016/0003-2697(81)90110-X

A computer program for determining the size of DNA restriction fragments

Ronald G. Duggleby, Howard Kinns, Julian I. Rood

Research output: Contribution to journal › Article › Research › peer-review

72 Citations (Scopus)

Abstract

A computer program has been developed for determining the sizes of DNA restriction fragments from their electrophoretic mobilities. A parabola is fitted to the mobilities of a set of standard fragments of known sizes and the sizes of the unknown fragments are then calculated from the fitted curve. This procedure is shown to yield estimated sizes which are accurate to within a few percentage, as judged by experiments with fragments obtained by digestion of pBR322 with the restriction endonuclease HaeIII. The program, which is written in BASIC, is simple to use and is very much faster than the graphical method that it replaces.

Original language	English
Pages (from-to)	49-55
Number of pages	7
Journal	Analytical Biochemistry
Volume	110
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(81)90110-X
Publication status	Published - 1 Jan 1981
Externally published	Yes

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A computer program for determining the size of DNA restriction fragments

Abstract

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