A computer program for determining the size of DNA restriction fragments

Ronald G. Duggleby, Howard Kinns, Julian I. Rood

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72 Citations (Scopus)


A computer program has been developed for determining the sizes of DNA restriction fragments from their electrophoretic mobilities. A parabola is fitted to the mobilities of a set of standard fragments of known sizes and the sizes of the unknown fragments are then calculated from the fitted curve. This procedure is shown to yield estimated sizes which are accurate to within a few percentage, as judged by experiments with fragments obtained by digestion of pBR322 with the restriction endonuclease HaeIII. The program, which is written in BASIC, is simple to use and is very much faster than the graphical method that it replaces.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalAnalytical Biochemistry
Issue number1
Publication statusPublished - 1 Jan 1981
Externally publishedYes

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