A comprehensive analysis of constitutive naturally processed and presented HLA-C*04:01 (Cw4)-specific peptides

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The human B lymphoblastoid cell line C1R is widely regarded as human leukocyte antigen-A (HLA-A)/HLA-B negative and is therefore frequently exploited as a recipient cell line to study HLA class I functions. However, the normal levels of HLA-C*04:01 often hamper the investigation of introduced HLA class I allomorphs, which is particularly evident in sensitive applications such as mass spectrometry. Here we describe the comprehensive analysis of endogenous HLA-C*04:01 ligands expressed on the surface of C1R cells to (i) define a large sequence dataset of HLA-C*04:01 ligands, to (ii) refine the HLA-C*04:01 peptide-binding motif and (iii) to provide a resource that allows discrimination between peptides bound to introduced HLA class I subtypes and to the endogenous HLA-C*04:01 molecules.
Original languageEnglish
Pages (from-to)174 - 179
Number of pages6
JournalTissue Antigens
Volume83
Issue number3
DOIs
Publication statusPublished - 2014

Cite this

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title = "A comprehensive analysis of constitutive naturally processed and presented HLA-C*04:01 (Cw4)-specific peptides",
abstract = "The human B lymphoblastoid cell line C1R is widely regarded as human leukocyte antigen-A (HLA-A)/HLA-B negative and is therefore frequently exploited as a recipient cell line to study HLA class I functions. However, the normal levels of HLA-C*04:01 often hamper the investigation of introduced HLA class I allomorphs, which is particularly evident in sensitive applications such as mass spectrometry. Here we describe the comprehensive analysis of endogenous HLA-C*04:01 ligands expressed on the surface of C1R cells to (i) define a large sequence dataset of HLA-C*04:01 ligands, to (ii) refine the HLA-C*04:01 peptide-binding motif and (iii) to provide a resource that allows discrimination between peptides bound to introduced HLA class I subtypes and to the endogenous HLA-C*04:01 molecules.",
author = "Schittenhelm, {Ralf Bernd} and Dudek, {Nadine Lee} and Croft, {Nathan Paul} and Sri Ramarathinam and Purcell, {Anthony Wayne}",
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doi = "10.1111/tan.12282",
language = "English",
volume = "83",
pages = "174 -- 179",
journal = "Tissue Antigens",
issn = "0001-2815",
publisher = "Wiley-Blackwell",
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}

A comprehensive analysis of constitutive naturally processed and presented HLA-C*04:01 (Cw4)-specific peptides. / Schittenhelm, Ralf Bernd; Dudek, Nadine Lee; Croft, Nathan Paul; Ramarathinam, Sri; Purcell, Anthony Wayne.

In: Tissue Antigens, Vol. 83, No. 3, 2014, p. 174 - 179.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A comprehensive analysis of constitutive naturally processed and presented HLA-C*04:01 (Cw4)-specific peptides

AU - Schittenhelm, Ralf Bernd

AU - Dudek, Nadine Lee

AU - Croft, Nathan Paul

AU - Ramarathinam, Sri

AU - Purcell, Anthony Wayne

PY - 2014

Y1 - 2014

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AB - The human B lymphoblastoid cell line C1R is widely regarded as human leukocyte antigen-A (HLA-A)/HLA-B negative and is therefore frequently exploited as a recipient cell line to study HLA class I functions. However, the normal levels of HLA-C*04:01 often hamper the investigation of introduced HLA class I allomorphs, which is particularly evident in sensitive applications such as mass spectrometry. Here we describe the comprehensive analysis of endogenous HLA-C*04:01 ligands expressed on the surface of C1R cells to (i) define a large sequence dataset of HLA-C*04:01 ligands, to (ii) refine the HLA-C*04:01 peptide-binding motif and (iii) to provide a resource that allows discrimination between peptides bound to introduced HLA class I subtypes and to the endogenous HLA-C*04:01 molecules.

UR - http://onlinelibrary.wiley.com/doi/10.1111/tan.12282/pdf

U2 - 10.1111/tan.12282

DO - 10.1111/tan.12282

M3 - Article

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SN - 0001-2815

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