A comparison of mesenchymal precursor cells and amnion epithelial cells for enhancing cervical interbody fusion in an ovine model

Tony Goldschlager, Peter Ghosh, Andrew Zannettino, Mark M Williamson, Jeffrey V Rosenfeld, Silviu Itescu, Graham Jenkin

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

BACKGROUND:: Rapid, reliable fusion is the goal in anterior cervical discectomy and fusion (ACDF). Iliac crest autograft has a high rate of donor site morbidity. Alternatives, such as bone graft substitutes, lack osteo-inductivity, and recombinant bone morphogenetic proteins risk life-threatening complications. Both allogeneic mesenchymal precursor cells (MPCs) and amnion derived epithelial cells (AECs) have osteogenic potential. OBJECTIVE:: To compare, for the first time, the capacity of MPCs and AECs to promote osteogenesis in an ovine model. METHODS:: Five groups of 2 year old ewes were subjected to C3/4 ACDF using a Fidji interbody cage packed with: A: Iliac crest autograft (AG) alone (n=6); B: Hydroxyapatite-tricalcium phosphate Mastergraft Granules (HA/TCP) alone (n=6); C: HA/TCP containing 5 million MPCs (n=6); D: HA/TCP containing 5 million AECs (n=5); E: age-matched non-operative controls (n=6). At 3 months, animals were sacrificed and quantitative multi-slice CT, functional radiography, biomechanics, histology, and histomorphometry were performed. RESULTS:: No procedural or cell related adverse events were observed. There was significantly more fusion in the MPC group (C) than in groups A, B, or D. CT scan at 3 months revealed 5/6 (83 ) MPC-treated animals had continuous bony bridging compared with 0/5 AEC and only 1/6 AG and 2/6 HA/TCP treated animals (p= 0.01). CONCLUSION:: Implantation of allogeneic MPCs in combination with HA/TCP within an interbody spacer facilitates interbody fusion following discectomy. The earlier, more robust fusion observed with MPCs, relative to autograft and HA/TCP bone substitute, indicates that this approach may offer a therapeutic benefit.
Original languageEnglish
Pages (from-to)1025 - 1034
Number of pages10
JournalNeurosurgery
Volume68
Issue number4
DOIs
Publication statusPublished - 2011

Cite this

@article{ebe04fba426f42b9a7d7efbd2bdedd98,
title = "A comparison of mesenchymal precursor cells and amnion epithelial cells for enhancing cervical interbody fusion in an ovine model",
abstract = "BACKGROUND:: Rapid, reliable fusion is the goal in anterior cervical discectomy and fusion (ACDF). Iliac crest autograft has a high rate of donor site morbidity. Alternatives, such as bone graft substitutes, lack osteo-inductivity, and recombinant bone morphogenetic proteins risk life-threatening complications. Both allogeneic mesenchymal precursor cells (MPCs) and amnion derived epithelial cells (AECs) have osteogenic potential. OBJECTIVE:: To compare, for the first time, the capacity of MPCs and AECs to promote osteogenesis in an ovine model. METHODS:: Five groups of 2 year old ewes were subjected to C3/4 ACDF using a Fidji interbody cage packed with: A: Iliac crest autograft (AG) alone (n=6); B: Hydroxyapatite-tricalcium phosphate Mastergraft Granules (HA/TCP) alone (n=6); C: HA/TCP containing 5 million MPCs (n=6); D: HA/TCP containing 5 million AECs (n=5); E: age-matched non-operative controls (n=6). At 3 months, animals were sacrificed and quantitative multi-slice CT, functional radiography, biomechanics, histology, and histomorphometry were performed. RESULTS:: No procedural or cell related adverse events were observed. There was significantly more fusion in the MPC group (C) than in groups A, B, or D. CT scan at 3 months revealed 5/6 (83 ) MPC-treated animals had continuous bony bridging compared with 0/5 AEC and only 1/6 AG and 2/6 HA/TCP treated animals (p= 0.01). CONCLUSION:: Implantation of allogeneic MPCs in combination with HA/TCP within an interbody spacer facilitates interbody fusion following discectomy. The earlier, more robust fusion observed with MPCs, relative to autograft and HA/TCP bone substitute, indicates that this approach may offer a therapeutic benefit.",
author = "Tony Goldschlager and Peter Ghosh and Andrew Zannettino and Williamson, {Mark M} and Rosenfeld, {Jeffrey V} and Silviu Itescu and Graham Jenkin",
year = "2011",
doi = "10.1227/NEU.0b013e31820d5375",
language = "English",
volume = "68",
pages = "1025 -- 1034",
journal = "Neurosurgery",
issn = "0148-396X",
publisher = "Lippincott Williams & Wilkins",
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A comparison of mesenchymal precursor cells and amnion epithelial cells for enhancing cervical interbody fusion in an ovine model. / Goldschlager, Tony; Ghosh, Peter; Zannettino, Andrew; Williamson, Mark M; Rosenfeld, Jeffrey V; Itescu, Silviu; Jenkin, Graham.

In: Neurosurgery, Vol. 68, No. 4, 2011, p. 1025 - 1034.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A comparison of mesenchymal precursor cells and amnion epithelial cells for enhancing cervical interbody fusion in an ovine model

AU - Goldschlager, Tony

AU - Ghosh, Peter

AU - Zannettino, Andrew

AU - Williamson, Mark M

AU - Rosenfeld, Jeffrey V

AU - Itescu, Silviu

AU - Jenkin, Graham

PY - 2011

Y1 - 2011

N2 - BACKGROUND:: Rapid, reliable fusion is the goal in anterior cervical discectomy and fusion (ACDF). Iliac crest autograft has a high rate of donor site morbidity. Alternatives, such as bone graft substitutes, lack osteo-inductivity, and recombinant bone morphogenetic proteins risk life-threatening complications. Both allogeneic mesenchymal precursor cells (MPCs) and amnion derived epithelial cells (AECs) have osteogenic potential. OBJECTIVE:: To compare, for the first time, the capacity of MPCs and AECs to promote osteogenesis in an ovine model. METHODS:: Five groups of 2 year old ewes were subjected to C3/4 ACDF using a Fidji interbody cage packed with: A: Iliac crest autograft (AG) alone (n=6); B: Hydroxyapatite-tricalcium phosphate Mastergraft Granules (HA/TCP) alone (n=6); C: HA/TCP containing 5 million MPCs (n=6); D: HA/TCP containing 5 million AECs (n=5); E: age-matched non-operative controls (n=6). At 3 months, animals were sacrificed and quantitative multi-slice CT, functional radiography, biomechanics, histology, and histomorphometry were performed. RESULTS:: No procedural or cell related adverse events were observed. There was significantly more fusion in the MPC group (C) than in groups A, B, or D. CT scan at 3 months revealed 5/6 (83 ) MPC-treated animals had continuous bony bridging compared with 0/5 AEC and only 1/6 AG and 2/6 HA/TCP treated animals (p= 0.01). CONCLUSION:: Implantation of allogeneic MPCs in combination with HA/TCP within an interbody spacer facilitates interbody fusion following discectomy. The earlier, more robust fusion observed with MPCs, relative to autograft and HA/TCP bone substitute, indicates that this approach may offer a therapeutic benefit.

AB - BACKGROUND:: Rapid, reliable fusion is the goal in anterior cervical discectomy and fusion (ACDF). Iliac crest autograft has a high rate of donor site morbidity. Alternatives, such as bone graft substitutes, lack osteo-inductivity, and recombinant bone morphogenetic proteins risk life-threatening complications. Both allogeneic mesenchymal precursor cells (MPCs) and amnion derived epithelial cells (AECs) have osteogenic potential. OBJECTIVE:: To compare, for the first time, the capacity of MPCs and AECs to promote osteogenesis in an ovine model. METHODS:: Five groups of 2 year old ewes were subjected to C3/4 ACDF using a Fidji interbody cage packed with: A: Iliac crest autograft (AG) alone (n=6); B: Hydroxyapatite-tricalcium phosphate Mastergraft Granules (HA/TCP) alone (n=6); C: HA/TCP containing 5 million MPCs (n=6); D: HA/TCP containing 5 million AECs (n=5); E: age-matched non-operative controls (n=6). At 3 months, animals were sacrificed and quantitative multi-slice CT, functional radiography, biomechanics, histology, and histomorphometry were performed. RESULTS:: No procedural or cell related adverse events were observed. There was significantly more fusion in the MPC group (C) than in groups A, B, or D. CT scan at 3 months revealed 5/6 (83 ) MPC-treated animals had continuous bony bridging compared with 0/5 AEC and only 1/6 AG and 2/6 HA/TCP treated animals (p= 0.01). CONCLUSION:: Implantation of allogeneic MPCs in combination with HA/TCP within an interbody spacer facilitates interbody fusion following discectomy. The earlier, more robust fusion observed with MPCs, relative to autograft and HA/TCP bone substitute, indicates that this approach may offer a therapeutic benefit.

UR - http://www.ncbi.nlm.nih.gov/pubmed/21242823

U2 - 10.1227/NEU.0b013e31820d5375

DO - 10.1227/NEU.0b013e31820d5375

M3 - Article

VL - 68

SP - 1025

EP - 1034

JO - Neurosurgery

JF - Neurosurgery

SN - 0148-396X

IS - 4

ER -