A common 43 K protein induced by glucocorticoids in a variety of cells and tissues

Nancy R. Nichols, B. A K Khalid, Peter J. Fuller, Barbara M. Rayson, John W. Funder

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Abstract

We have investigated the domain of adrenal steriod action in a variety of mammalian cells and tissue by high resolution two-dimensional gel electrophoresis and autoradiography. Following in vivo or in vitro treatment with steroids, minced tissue or cells were pulsed with [35S]methionine, and they newly synthesized polypeptides compared with controls by visual inspection of partial protein maps. We have found a similar protein (Mr ∼ 41-44K, pI ∼ 6.3-6.5) to be consistently increased by dexamethasone in 3 rat tissues and 9 human, bovine and rat cell lines. The protein was constitutively synthesized in all targets; quantitative measurements on the magnitude of response show a 2-fold induction by dexamethasone in all systems, in vivo and in vitro. Glucocorticoid-specific hormones but not sex steroids increase the rate of synthesis; deoxycorticosterone has agonist or antagonist effects on 43K synthesis in different systems studied. Co-electrophoresis experiments indicated that 43K proteins co-migrate in at least 3 rat cell lines from tissues with diverse functions. The functional significance of this common glucocorticoid-induced protein is unknown; its ubiquity and electrophoretic properties suggest a highly conserved, common indicator of glucocorticoid action in diverse target cells and tissues, rather than a tissue-specific response.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume37
Issue number2
DOIs
Publication statusPublished - 1984
Externally publishedYes

Keywords

  • co-electrophoresis
  • dexamethasone
  • protein synthesis
  • steroid response
  • two-dimensional electrophoresis

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