A combined physical and genetic map of Pseudomonas aeruginosa PAO

E. Ratnaningsih, S. Dharmsthiti, V. Krishnapillai, A. Morgan, M. Sinclair, B. W. Holloway

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Abstract

A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.

Original languageEnglish
Pages (from-to)2351-2357
Number of pages7
JournalJournal of General Microbiology
Volume136
Issue number12
DOIs
Publication statusPublished - 1 Jan 1990

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