A combined physical and genetic map of Pseudomonas aeruginosa PAO

E. Ratnaningsih; S. Dharmsthiti; V. Krishnapillai; A. Morgan; M. Sinclair; B. W. Holloway

doi:10.1099/00221287-136-12-2351

A combined physical and genetic map of Pseudomonas aeruginosa PAO

E. Ratnaningsih, S. Dharmsthiti, V. Krishnapillai, A. Morgan, M. Sinclair, B. W. Holloway

Anatomy & Developmental Biology

Research output: Contribution to journal › Article › Research › peer-review

52 Citations (Scopus)

Abstract

A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.

Original language	English
Pages (from-to)	2351-2357
Number of pages	7
Journal	Journal of General Microbiology
Volume	136
Issue number	12
DOIs	https://doi.org/10.1099/00221287-136-12-2351
Publication status	Published - 1 Jan 1990

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10.1099/00221287-136-12-2351

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Cite this

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