A chick embryo fibroblast protein kinase recognizing ribosomal protein S6. Activity increase after serum stimulation

Alfons Lawen; Oskar H W Martini

doi:10.1016/0014-5793(85)80921-2

A chick embryo fibroblast protein kinase recognizing ribosomal protein S6. Activity increase after serum stimulation

Alfons Lawen, Oskar H W Martini

Biochemistry & Molecular Biology

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Abstract

We sought protein kinase(ser) activity in DEAE-Sephacel chromatography fractions of the 10000 × g supernatants of chick embryo fibroblasts using 40 S ribosomal subunits as kinase substrate, and detected a new S6-recognizing kinase activity. There was one order of magnitude more enzyme activity in chromatography fractions derived from serum-stimulated than from serum-deprived cells. Known protein kinase regulators and a low dose tryptic treatment did not increase the enzyme's activity. Protein kinase Ribosomal protein S6 Chick embryo fibroblast Growth control.

Original language	English
Pages (from-to)	272-276
Number of pages	5
Journal	FEBS Letters
Volume	185
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80921-2
Publication status	Published - 17 Jun 1985

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abstract = "We sought protein kinase(ser) activity in DEAE-Sephacel chromatography fractions of the 10000 × g supernatants of chick embryo fibroblasts using 40 S ribosomal subunits as kinase substrate, and detected a new S6-recognizing kinase activity. There was one order of magnitude more enzyme activity in chromatography fractions derived from serum-stimulated than from serum-deprived cells. Known protein kinase regulators and a low dose tryptic treatment did not increase the enzyme's activity. Protein kinase Ribosomal protein S6 Chick embryo fibroblast Growth control.",

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AB - We sought protein kinase(ser) activity in DEAE-Sephacel chromatography fractions of the 10000 × g supernatants of chick embryo fibroblasts using 40 S ribosomal subunits as kinase substrate, and detected a new S6-recognizing kinase activity. There was one order of magnitude more enzyme activity in chromatography fractions derived from serum-stimulated than from serum-deprived cells. Known protein kinase regulators and a low dose tryptic treatment did not increase the enzyme's activity. Protein kinase Ribosomal protein S6 Chick embryo fibroblast Growth control.

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A chick embryo fibroblast protein kinase recognizing ribosomal protein S6. Activity increase after serum stimulation

Abstract

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