A chick embryo fibroblast protein kinase recognizing ribosomal protein S6. Activity increase after serum stimulation

Alfons Lawen, Oskar H W Martini

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We sought protein kinase(ser) activity in DEAE-Sephacel chromatography fractions of the 10000 × g supernatants of chick embryo fibroblasts using 40 S ribosomal subunits as kinase substrate, and detected a new S6-recognizing kinase activity. There was one order of magnitude more enzyme activity in chromatography fractions derived from serum-stimulated than from serum-deprived cells. Known protein kinase regulators and a low dose tryptic treatment did not increase the enzyme's activity. Protein kinase Ribosomal protein S6 Chick embryo fibroblast Growth control.

Original languageEnglish
Pages (from-to)272-276
Number of pages5
JournalFEBS Letters
Issue number2
Publication statusPublished - 17 Jun 1985

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