A cDNA clone expressing a rhoptry protein of Plasmodium falciparum

Ross L. Coppel; A. Edward Bianco; Janetta G. Culvenor; Pauline E. Crewther; Graham V. Brown; Robin F. Anders; David J. Kemp

doi:10.1016/0166-6851(87)90020-X

A cDNA clone expressing a rhoptry protein of Plasmodium falciparum

Ross L. Coppel, A. Edward Bianco, Janetta G. Culvenor, Pauline E. Crewther, Graham V. Brown, Robin F. Anders, David J. Kemp

Research output: Contribution to journal › Article › Research › peer-review

39 Citations (Scopus)

Abstract

Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.

Original language	English
Pages (from-to)	73-81
Number of pages	9
Journal	Molecular and Biochemical Parasitology
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/0166-6851(87)90020-X
Publication status	Published - 1 Jan 1987
Externally published	Yes

Keywords

DNA sequence
Gene cloning
Immunoelectronmicroscopy
Plasmodium falciparum
Rhoptry antigen

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10.1016/0166-6851(87)90020-X

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abstract = "Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.",

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AU - Coppel, Ross L.

AU - Bianco, A. Edward

AU - Culvenor, Janetta G.

AU - Crewther, Pauline E.

AU - Brown, Graham V.

AU - Anders, Robin F.

AU - Kemp, David J.

PY - 1987/1/1

Y1 - 1987/1/1

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AB - Antibodies from immune humans were used to select a cDNA clone expressing an asexual blood stage antigen of Plasmodium falciparum. The expressed fused polypeptide was used as an affinity reagent to purify human antibodies specific for the corresponding parasite antigen. Western blotting and immunoelectronmicroscopy demonstrated that the antigen was a 105 kDa protein located in the rhoptries of merozoites. The cDNA encodes the carboxy terminus of the rhoptry antigen, a sequence rich both in charged and hydroxy amino acids.

KW - DNA sequence

KW - Gene cloning

KW - Immunoelectronmicroscopy

KW - Plasmodium falciparum

KW - Rhoptry antigen

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JF - Molecular and Biochemical Parasitology

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ER -

A cDNA clone expressing a rhoptry protein of Plasmodium falciparum

Abstract

Keywords

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