A Carborane-Containing Fluorophore as a Stain of Cellular Lipid Droplets

Andrew Wu, Jacek L. Kolanowski, Badwi B. Boumelhem, Kylie Yang, Rebecca Lee, Amandeep Kaur, Stuart T. Fraser, Elizabeth J. New, Louis M. Rendina

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30 Citations (Scopus)


The use of fluorescent markers and probes greatly enhances biological investigations but relies on the provision of an array of fluorophores with diverse properties. Herein we report a novel carborane-containing coumarin, 5, which is sufficiently lipophilic to localise in cellular lipid droplets. In non-polar solvents which show comparable polarities to those of a lipid environment, compound 5 exhibits a fluorescence quantum yield two orders of magnitude greater than found in aqueous solvents, adding a further degree of selectivity to lipid droplet imaging. Compound 5 can stain lipid droplets in ex vivo adipocytes as well as in cultured cells, and can be utilised in flow cytometry as well as confocal microscopy.

Original languageEnglish
Pages (from-to)1704-1708
Number of pages5
JournalChemistry - An Asian Journal
Issue number14
Publication statusPublished - 18 Jul 2017
Externally publishedYes


  • carboranes
  • cellular markers
  • coumarins
  • fluorophores
  • lipid droplets

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