Ion channels that regulate ion permeation through cell membranes are very important for the implementation of various significant physiological functions in life processes. Numerous kinds of metal ions are physiologically indispensable and play crucial roles in living systems. Metal-ion channels often regulate the transport of ions or molecules across cell membranes by the conformational changes of the channel proteins in response to various kinds of stimuli, such as potassium and zinc ions. As a carrier of ion transport, ion channels will have great applications in the field of biotechnology, such as biosensors, controlling nanofluids, and drug delivery systems. The membrane was soaked in MilliQ water to remove residual salts. The diameter of the large opening of the conical nanochannels which was called base and the narrow side which was called tip was determined by scanning electron microscopy.