A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene

Heegyum Moon, Sunghee Cho, Tiing Jen Loh, Jianhua Zhou, Claudia Ghigna, Giuseppe Biamonti, Michael R. Green, Xuexiu Zheng, Haihong Shen

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2 Citations (Scopus)

Abstract

Ron is a human receptor for the macrophage-stimulating protein (MSP). Exon 11 skipping of Ron pre-mRNA produces the RonΔ165 protein that has a deletion of a 49 amino acid region in the β-chain extracellular domain. RonΔ165 is constitutively active even in the absence of its ligand. Through stepwise deletion analysis, we identified a 2-nt RNA enhancer, which is located 74 nt upstream from the 5′ splice site of exon 11, for exon 11 inclusion. Through double-base and single-base substitution analysis of the 2-nt RNA, we demonstrated that the GA, CC, UG and AC dinucleotides on exon 11, in addition to the wild-type AG sequence, function as enhancers for exon 11 inclusion of the Ron pre-mRNA.

Original languageEnglish
Pages (from-to)450-455
Number of pages6
JournalOncology Reports
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 2014
Externally publishedYes

Keywords

  • Cancer
  • cis element
  • Exon 11 inclusion
  • Pre-mRNA splicing
  • Proto-oncogene
  • Ron

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