6-[N,S-dimethyl-N'-cyanothioureidomethyl]-6,11-dihydro-5H-dibenz[b,e]a zepine hydrochloride (Fran 12): A histamine and 5-hydroxytryptamine antagonist with pressor properties

S. C. Law, F. J. Guyett, R. G. King, A. L A Boura, W. R. Jackson, W. C. Hodgson

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Abstract

We have synthesized and examined some of the pharmacological properties of 6-[N,S-dimethyl-N'-cyanoisothioureidomethyl]-6,11-dihydro-5H-dibenz(b, e)azepine hydrochloride (Fran 12), a derivative of 6-methylaminomethyl-6,11-dihydro-5H-dibenz[b,e,]azepine. In the guinea-pig isolated ileum, Fran 12 (10-7-10-5 M) caused parallel rightward shifts of the concentration-response curves to histamine. A Schild plot gave a pA2 of 7.48, with a slope not significantly different from -1.0. In the rat stomach fundus strip and in endothelium-denuded aortic rings, Fran 12 inhibited contractile responses to 5-hydroxytryptamine in a noncompetitive manner. In both chloralose-anaesthetized and pithed rats, it inhibited pressor responses to 5-hydroxytryptamine. It had no effect on depressor responses to 5-hydroxytryptamine in anaesthetized rats. In pithed rats, Fran 12 (0.25-2 mg/kg, i.v.) produced dose-dependent increases in blood pressure. These were not inhibited by i.v. phentolamine, prazosin, yohimbine, propranolol, methysergide, pentolinium or atropine but were inhibited by verapamil. These results indicate that Fran 12 is a histamine and 5-hydroxytryptamine antagonist which also exerts pressor effects via a peripheral action. The pressor action does not appear to be mediated via effects on α1- or α2-adrenoceptors, muscarinic or nicotinic cholinoceptors or 5-hydroxytryptamine receptors, although calcium channel activation may play a role.

Original languageEnglish
Pages (from-to)67-80
Number of pages14
JournalArchives Internationales de Pharmacodynamie et de Therapie
Volume317
Publication statusPublished - 1 Jan 1992

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