Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 616 - 625 |
| Number of pages | 10 |
| Journal | Chemical Biology & Drug Design |
| Volume | 84 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2014 |
Cite this
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19F NMR as a probe of ligand interactions with the iNOS binding site of SPRY domain-containing SOCS box protein 2. / Leung, Eleanor Wai Wai; Yagi, Hiromasa; Harjani, Jitendra Rameshlal; Mulcair, Mark; Scanlon, Martin; Baell, Jonathan Bayldon; Norton, Raymond Stanley.
In: Chemical Biology & Drug Design, Vol. 84, No. 5, 2014, p. 616 - 625.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - 19F NMR as a probe of ligand interactions with the iNOS binding site of SPRY domain-containing SOCS box protein 2
AU - Leung, Eleanor Wai Wai
AU - Yagi, Hiromasa
AU - Harjani, Jitendra Rameshlal
AU - Mulcair, Mark
AU - Scanlon, Martin
AU - Baell, Jonathan Bayldon
AU - Norton, Raymond Stanley
PY - 2014
Y1 - 2014
N2 - SPRY domain-containing SOCS box protein 2 (SPSB2) regulates inducible nitric oxide synthase (iNOS) by targeting it for proteasomal degradation. Inhibiting this interaction prolongs the intracellular lifetime of iNOS, leading in turn to enhanced killing of infectious pathogens such as bacteria and parasites. SPSB2 recognizes a linear motif (DINNN) in the disordered N-terminus of iNOS, and ligands that target the DINNN binding site on SPSB2 are potentially novel anti-infective agents. We have explored 19F NMR as a means of probing ligand binding to SPSB2. All six Trp residues in SPSB2 were replaced with 5-fluorotryptophan (5-F-Trp) by utilizing a Trp auxotroph strain of Escherichia coli . The labeled protein was well folded and bound a DINNN-containing peptide with similar affinity to native SPSB2. Six well-resolved 5-F-Trp resonances were observed in the 19F NMR spectrum and were assigned using site-directed mutagenesis. The 19F resonance of W207 was significantly perturbed upon binding to DINNN-containing peptides. Other resonances were perturbed to a lesser extent although in a way that was sensitive to the composition of the peptide. Analogues of compounds identified in a fragment screen also perturbed the W207 resonance, confirming their binding to the iNOS peptide-binding site on SPSB2. 19F NMR promises to be a valuable approach in developing inhibitors that bind to the DINNN binding site.
AB - SPRY domain-containing SOCS box protein 2 (SPSB2) regulates inducible nitric oxide synthase (iNOS) by targeting it for proteasomal degradation. Inhibiting this interaction prolongs the intracellular lifetime of iNOS, leading in turn to enhanced killing of infectious pathogens such as bacteria and parasites. SPSB2 recognizes a linear motif (DINNN) in the disordered N-terminus of iNOS, and ligands that target the DINNN binding site on SPSB2 are potentially novel anti-infective agents. We have explored 19F NMR as a means of probing ligand binding to SPSB2. All six Trp residues in SPSB2 were replaced with 5-fluorotryptophan (5-F-Trp) by utilizing a Trp auxotroph strain of Escherichia coli . The labeled protein was well folded and bound a DINNN-containing peptide with similar affinity to native SPSB2. Six well-resolved 5-F-Trp resonances were observed in the 19F NMR spectrum and were assigned using site-directed mutagenesis. The 19F resonance of W207 was significantly perturbed upon binding to DINNN-containing peptides. Other resonances were perturbed to a lesser extent although in a way that was sensitive to the composition of the peptide. Analogues of compounds identified in a fragment screen also perturbed the W207 resonance, confirming their binding to the iNOS peptide-binding site on SPSB2. 19F NMR promises to be a valuable approach in developing inhibitors that bind to the DINNN binding site.
UR - http://onlinelibrary.wiley.com/doi/10.1111/cbdd.12355/epdf
U2 - 10.1111/cbdd.12355
DO - 10.1111/cbdd.12355
M3 - Article
VL - 84
SP - 616
EP - 625
JO - Chemical Biology & Drug Design
JF - Chemical Biology & Drug Design
SN - 1747-0277
IS - 5
ER -