17β-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells

Zhong-Lin Chai, Phillip Brereton, Takashi Suzuki, Hironobu Sasano, Varuni Obeyesekere, Genevieve Escher, Richard Saffery, Peter Fuller, Carla Enriquez, Zygmunt Krozowski

Research output: Contribution to journalArticleResearchpeer-review

49 Citations (Scopus)

Abstract

We searched expressed sequence tag databases with conserved domains of the short-chain alcohol dehydrogenase superfamily and identified another isoform of 17β-hydroxysteroid dehydrogenase, 17βHSDXI. This enzyme converts 5α-androstane-3α, 17β-diol to androsterone. The substrate has been implicated in supporting gestation and modulating γ-aminobutyric acid receptor activity, 17βHSDXI is colinear with human retinal short-chain dehydrogenase/reductase retSDR2, a protein with no known biological activity (accession no. AAF06939). Of the proteins with known function, 17βHSDXI is most closely related to the retinol-metabolizing enzyme retSDR1, with which it has 30% identity. There is a polymorphic stretch of 15 adenosines in the 5′ untranslated region of the cDNA sequence and a silent polymorphism at C719T. A 17βHSDXI construct with a stretch of 20 adenosines was found to produce significantly more enzyme activity than constructs containing 15 or less adenosines (43% vs. 26%, P

Original languageEnglish
Pages (from-to)2084-2091
Number of pages8
JournalEndocrinology
Volume144
Issue number5
DOIs
Publication statusPublished - 1 May 2003
Externally publishedYes

Cite this

Chai, Z-L., Brereton, P., Suzuki, T., Sasano, H., Obeyesekere, V., Escher, G., ... Krozowski, Z. (2003). 17β-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells. Endocrinology, 144(5), 2084-2091. https://doi.org/10.1210/en.2002-221030
Chai, Zhong-Lin ; Brereton, Phillip ; Suzuki, Takashi ; Sasano, Hironobu ; Obeyesekere, Varuni ; Escher, Genevieve ; Saffery, Richard ; Fuller, Peter ; Enriquez, Carla ; Krozowski, Zygmunt. / 17β-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells. In: Endocrinology. 2003 ; Vol. 144, No. 5. pp. 2084-2091.
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abstract = "We searched expressed sequence tag databases with conserved domains of the short-chain alcohol dehydrogenase superfamily and identified another isoform of 17β-hydroxysteroid dehydrogenase, 17βHSDXI. This enzyme converts 5α-androstane-3α, 17β-diol to androsterone. The substrate has been implicated in supporting gestation and modulating γ-aminobutyric acid receptor activity, 17βHSDXI is colinear with human retinal short-chain dehydrogenase/reductase retSDR2, a protein with no known biological activity (accession no. AAF06939). Of the proteins with known function, 17βHSDXI is most closely related to the retinol-metabolizing enzyme retSDR1, with which it has 30{\%} identity. There is a polymorphic stretch of 15 adenosines in the 5′ untranslated region of the cDNA sequence and a silent polymorphism at C719T. A 17βHSDXI construct with a stretch of 20 adenosines was found to produce significantly more enzyme activity than constructs containing 15 or less adenosines (43{\%} vs. 26{\%}, P",
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Chai, Z-L, Brereton, P, Suzuki, T, Sasano, H, Obeyesekere, V, Escher, G, Saffery, R, Fuller, P, Enriquez, C & Krozowski, Z 2003, '17β-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells', Endocrinology, vol. 144, no. 5, pp. 2084-2091. https://doi.org/10.1210/en.2002-221030

17β-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells. / Chai, Zhong-Lin; Brereton, Phillip; Suzuki, Takashi; Sasano, Hironobu; Obeyesekere, Varuni; Escher, Genevieve; Saffery, Richard; Fuller, Peter; Enriquez, Carla; Krozowski, Zygmunt.

In: Endocrinology, Vol. 144, No. 5, 01.05.2003, p. 2084-2091.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Chai, Zhong-Lin

AU - Brereton, Phillip

AU - Suzuki, Takashi

AU - Sasano, Hironobu

AU - Obeyesekere, Varuni

AU - Escher, Genevieve

AU - Saffery, Richard

AU - Fuller, Peter

AU - Enriquez, Carla

AU - Krozowski, Zygmunt

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Chai Z-L, Brereton P, Suzuki T, Sasano H, Obeyesekere V, Escher G et al. 17β-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells. Endocrinology. 2003 May 1;144(5):2084-2091. https://doi.org/10.1210/en.2002-221030