清开灵和双黄连口服液体内抗禽流感病毒作用

Objective: To evaluate the activity of Qingkailing and Shuanghuanglian Orals against avian influenza virus in vivo, and investigate the effect of the two Chinese medicinal formulas on immunologic function of infected mice. Methods: The BALB/c mouse model with viral pneumonia by avian influenza H9N2 virus was adopted. Protective effects of Qingkailing and Shuanghuanglian Orals against H9N2 virus in mice were investigated based on the lung index inhibitory rate, life-protection rate, and virus titer in lungs. Furthermore, the immunologic functions of the two Chinese medicinal formulas on infected mice were investigated based on the spleen index, the thymus index, and the percentage of T cell subgroups (CD4⁺/CD8⁺). Results: Qingkailing Oral, as well as Shuanghuanglian Oral, significantly inhibited the lung consolidation, improved the survival rate, and reduced the virus titer on mice infected with H9N2 virus. The lung index inhibitory rates were 34.1% and 26.3%, and the life-protection rates were 70.0% and 60.0%, respectively. Whereas, life-protection rate of the viral control group was 30.0%. Qingkailing and Shuanghuanglian Orals significantly exhibited the prevention against atrophy of spleen and thymus of mice caused by viral infection, and improved the percentage of CD4⁺/CD8⁺ T cells in spleen of infected mice. Conclusion: Qingkailing and Shuanghuanglian Orals have ideal efficacy against influenza virus in vivo. Inhibition on viral multiplication and adjustment for deviant immunologic function of infected mice are verified as the mechanism of action of the two Chinese medicinal formulae against influenza virus.