γH2AX as a molecular marker of aging and disease

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Abstract

Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalEpigenetics
Volume5
Issue number2
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Aging
  • Carcinogenesis
  • Chromatin modification
  • DNA damage
  • Double-strand break
  • Repair
  • γH2AX

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