γH2AX as a molecular marker of aging and disease

Research output: Contribution to journalReview ArticleResearchpeer-review

Abstract

Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalEpigenetics
Volume5
Issue number2
DOIs
Publication statusPublished - 2010
Externally publishedYes

Keywords

  • Aging
  • Carcinogenesis
  • Chromatin modification
  • DNA damage
  • Double-strand break
  • Repair
  • γH2AX

Cite this

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abstract = "Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.",
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γH2AX as a molecular marker of aging and disease. / Mah, Li Jeen; El-Osta, Assam; Karagiannis, Tom C.

In: Epigenetics, Vol. 5, No. 2, 2010, p. 129-136.

Research output: Contribution to journalReview ArticleResearchpeer-review

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AU - El-Osta, Assam

AU - Karagiannis, Tom C.

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KW - Carcinogenesis

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