Abstract
Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.
| Original language | English |
|---|---|
| Pages (from-to) | 129-136 |
| Number of pages | 8 |
| Journal | Epigenetics |
| Volume | 5 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 |
| Externally published | Yes |
Keywords
- Aging
- Carcinogenesis
- Chromatin modification
- DNA damage
- Double-strand break
- Repair
- γH2AX
Cite this
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γH2AX as a molecular marker of aging and disease. / Mah, Li Jeen; El-Osta, Assam; Karagiannis, Tom C.
In: Epigenetics, Vol. 5, No. 2, 2010, p. 129-136.Research output: Contribution to journal › Review Article › Research › peer-review
TY - JOUR
T1 - γH2AX as a molecular marker of aging and disease
AU - Mah, Li Jeen
AU - El-Osta, Assam
AU - Karagiannis, Tom C.
PY - 2010
Y1 - 2010
N2 - Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.
AB - Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.
KW - Aging
KW - Carcinogenesis
KW - Chromatin modification
KW - DNA damage
KW - Double-strand break
KW - Repair
KW - γH2AX
UR - http://www.scopus.com/inward/record.url?scp=77954022035&partnerID=8YFLogxK
U2 - 10.4161/epi.5.2.11080
DO - 10.4161/epi.5.2.11080
M3 - Review Article
VL - 5
SP - 129
EP - 136
JO - Epigenetics
JF - Epigenetics
SN - 1559-2294
IS - 2
ER -