Abstract
Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.
Original language | English |
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Pages (from-to) | 129-136 |
Number of pages | 8 |
Journal | Epigenetics |
Volume | 5 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2010 |
Externally published | Yes |
Keywords
- Aging
- Carcinogenesis
- Chromatin modification
- DNA damage
- Double-strand break
- Repair
- γH2AX