γH2AX as a molecular marker of aging and disease

Li Jeen Mah; Assam El-Osta; Tom C. Karagiannis

doi:10.4161/epi.5.2.11080

γH2AX as a molecular marker of aging and disease

Li Jeen Mah, Assam El-Osta, Tom C. Karagiannis

Research output: Contribution to journal › Review Article › Research › peer-review

89 Citations (Scopus)

Abstract

Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.

Original language	English
Pages (from-to)	129-136
Number of pages	8
Journal	Epigenetics
Volume	5
Issue number	2
DOIs	https://doi.org/10.4161/epi.5.2.11080
Publication status	Published - 2010
Externally published	Yes

Keywords

Aging
Carcinogenesis
Chromatin modification
DNA damage
Double-strand break
Repair
γH2AX

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4161/epi.5.2.11080

Cite this

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abstract = "Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.",

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AU - Karagiannis, Tom C.

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AB - Double-strand breaks are one of the most critical DNA lesions with respect to cell-death and preservation of genomic integrity. Rapid phosphorylation of the histone variant H2AX at Ser-139 to form γH2AX is an early cellular response to DNA double-strand breaks. Visualization of discrete γH2AX foci using immunofluorescence-based assays has provided a sensitive and effective method for detecting DSBs which may be implicated in various pathologies including cancer, age-related diseases, chronic inflammatory diseases and ischemia-reperfusion injury. In this review, the potential utility and significance of γH2AX as a molecular marker of aging and disease is analysed.

KW - Aging

KW - Carcinogenesis

KW - Chromatin modification

KW - DNA damage

KW - Double-strand break

KW - Repair

KW - γH2AX

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M3 - Review Article

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JO - Epigenetics

JF - Epigenetics

SN - 1559-2294

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ER -

γH2AX as a molecular marker of aging and disease

Abstract

Keywords

UN SDGs

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