β3-Tripeptides Coassemble into Fluorescent Hydrogels for Serial Monitoring in Vivo

Ketav Kulkarni, Juichien Hung, Alex J. Fulcher, Alex H.P. Chan, Andrew Hong, John S. Forsythe, Marie-Isabel Aguilar, Steven G. Wise, Mark P. Del Borgo

Research output: Contribution to journalLetterResearchpeer-review

7 Citations (Scopus)

Abstract

β3-peptides uniquely form shear thinning hydrogels which are proteolytically stable and biocompatible. Herein we describe the synthesis, material and optical characterization of a new class of fluorescently labeled hydrogelators based on a helical N-acetylated β3-peptide backbone. The resulting hydrogels were analyzed using fluorescence microscopy to confirm successful incorporation of the fluorophore within the fiber matrix without compromising the β3-peptide self-assembly. Serial, noninvasive conscious animal imaging was used to monitor the injected hydrogel, delivered via subcutaneous injection, while tracking their degradation patterns in real-time. The hydrogels demonstrated persistent, high-intensity fluorescence when monitored over a 14-day period.

Original languageEnglish
Pages (from-to)3843-3847
Number of pages5
JournalACS Biomaterials Science & Engineering
Volume4
Issue number11
DOIs
Publication statusPublished - 2 Oct 2018

Keywords

  • fluorescence
  • hydrogels
  • peptide biomaterials
  • self-assembly

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