α2-adrenoceptors in dog kidney: Autoradiographic localization and putative functions

R. J. Summers; J. A. Stephenson; S. Lipe; C. B. Neylon

α2-adrenoceptors in dog kidney: Autoradiographic localization and putative functions

R. J. Summers, J. A. Stephenson, S. Lipe, C. B. Neylon

Drug Discovery Biology

Research output: Contribution to journal › Article › Research › peer-review

9 Citations (Scopus)

Abstract

1. The location of α₂-adrenoceptors has been examined in slide-mounted sections of dog kidney by using labelling in vitro with [³H]rauwolscine and autoradiography. 2. [³H]Rauwolscine binding in dog kidney was of high affinity, saturable, and to a single population of sites. Stereoselectivity was observed for (-)-noradrenaline and the relative affinity of a series of competing antagonists indicated binding to α₂-adrenoceptors. 3. Autoradiography of [³H]rauwolscine binding revealed localization to three areas: glomeruli, the intimal and adventitial surfaces of arcuate blood vessels and the medullary vascular bundles.

Original language	English
Journal	Clinical Science
Volume	68
Issue number	SUPPL. 10
Publication status	Published - 1 Jan 1985

Cite this

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title = "α2-adrenoceptors in dog kidney: Autoradiographic localization and putative functions",

abstract = "1. The location of α2-adrenoceptors has been examined in slide-mounted sections of dog kidney by using labelling in vitro with [3H]rauwolscine and autoradiography. 2. [3H]Rauwolscine binding in dog kidney was of high affinity, saturable, and to a single population of sites. Stereoselectivity was observed for (-)-noradrenaline and the relative affinity of a series of competing antagonists indicated binding to α2-adrenoceptors. 3. Autoradiography of [3H]rauwolscine binding revealed localization to three areas: glomeruli, the intimal and adventitial surfaces of arcuate blood vessels and the medullary vascular bundles.",

author = "Summers, {R. J.} and Stephenson, {J. A.} and S. Lipe and Neylon, {C. B.}",

year = "1985",

month = jan,

day = "1",

language = "English",

volume = "68",

journal = "Clinical Science",

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T1 - α2-adrenoceptors in dog kidney

T2 - Autoradiographic localization and putative functions

AU - Summers, R. J.

AU - Stephenson, J. A.

AU - Lipe, S.

AU - Neylon, C. B.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - 1. The location of α2-adrenoceptors has been examined in slide-mounted sections of dog kidney by using labelling in vitro with [3H]rauwolscine and autoradiography. 2. [3H]Rauwolscine binding in dog kidney was of high affinity, saturable, and to a single population of sites. Stereoselectivity was observed for (-)-noradrenaline and the relative affinity of a series of competing antagonists indicated binding to α2-adrenoceptors. 3. Autoradiography of [3H]rauwolscine binding revealed localization to three areas: glomeruli, the intimal and adventitial surfaces of arcuate blood vessels and the medullary vascular bundles.

AB - 1. The location of α2-adrenoceptors has been examined in slide-mounted sections of dog kidney by using labelling in vitro with [3H]rauwolscine and autoradiography. 2. [3H]Rauwolscine binding in dog kidney was of high affinity, saturable, and to a single population of sites. Stereoselectivity was observed for (-)-noradrenaline and the relative affinity of a series of competing antagonists indicated binding to α2-adrenoceptors. 3. Autoradiography of [3H]rauwolscine binding revealed localization to three areas: glomeruli, the intimal and adventitial surfaces of arcuate blood vessels and the medullary vascular bundles.

UR - http://www.scopus.com/inward/record.url?scp=0021959069&partnerID=8YFLogxK

M3 - Article

C2 - 2982536

AN - SCOPUS:0021959069

VL - 68

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

IS - SUPPL. 10

ER -

α2-adrenoceptors in dog kidney: Autoradiographic localization and putative functions

Abstract

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