α-N-acetylated β-endorphin is differentially processed in the normal and hypothalamo-pituitary-disconnected (HPD) ewe

A. I. Smith; C. A. Wallace; D. J. Autelitano; M. C. Cheng; I. J. Clarke; J. W. Funder

doi:10.1016/0304-3940(86)90310-1

α-N-acetylated β-endorphin is differentially processed in the normal and hypothalamo-pituitary-disconnected (HPD) ewe

A. I. Smith, C. A. Wallace, D. J. Autelitano, M. C. Cheng, I. J. Clarke, J. W. Funder

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Abstract

We have used specific radioimmunoassay and high-performance liquid chromatography (HPLC) to determine content and molecular forms of pituitary α-N-acetylated endorphin (NacEP) in the intact and hypothalamo-pituitary-disconnected (HPD) sheep. No significant differences were seen in anterior pituitary (AP) immunoreactive (ir-) NacEP levels (control=4.34±0.45 ng/mg; HPD=4.38±0.83 ng/mg; n=4) or in HPLC profiles following pituitary disconnection. In contrast, levels of ir-NacEP in the HPD intermediate lobe (IL) (263±16.5 ng/mg; n=4) were double those in the control (110±14 ng/mg; n=4), accompanied by a marked change in the molecular profile. In the control IL, the major species were NacαEP (30%), NacγEP (30%) and NacβEP_1-27 (35%); in HPD IL, NacβEP_1-27 (65%) is the dominant molecular form, with NacαEP (11%) and NacγEP (9%) relatively minor components.

Original language	English
Pages (from-to)	229-233
Number of pages	5
Journal	Neuroscience Letters
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(86)90310-1
Publication status	Published - 11 Apr 1986
Externally published	Yes

Keywords

acetylation
dopamine
high-performance liquid chromatography
processing
radioimmunoassay
sheep pituitary
β-endorphin

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title = "α-N-acetylated β-endorphin is differentially processed in the normal and hypothalamo-pituitary-disconnected (HPD) ewe",

abstract = "We have used specific radioimmunoassay and high-performance liquid chromatography (HPLC) to determine content and molecular forms of pituitary α-N-acetylated endorphin (NacEP) in the intact and hypothalamo-pituitary-disconnected (HPD) sheep. No significant differences were seen in anterior pituitary (AP) immunoreactive (ir-) NacEP levels (control=4.34±0.45 ng/mg; HPD=4.38±0.83 ng/mg; n=4) or in HPLC profiles following pituitary disconnection. In contrast, levels of ir-NacEP in the HPD intermediate lobe (IL) (263±16.5 ng/mg; n=4) were double those in the control (110±14 ng/mg; n=4), accompanied by a marked change in the molecular profile. In the control IL, the major species were NacαEP (30%), NacγEP (30%) and NacβEP1-27 (35%); in HPD IL, NacβEP1-27 (65%) is the dominant molecular form, with NacαEP (11%) and NacγEP (9%) relatively minor components.",

keywords = "acetylation, dopamine, high-performance liquid chromatography, processing, radioimmunoassay, sheep pituitary, β-endorphin",

author = "Smith, {A. I.} and Wallace, {C. A.} and Autelitano, {D. J.} and Cheng, {M. C.} and Clarke, {I. J.} and Funder, {J. W.}",

year = "1986",

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T1 - α-N-acetylated β-endorphin is differentially processed in the normal and hypothalamo-pituitary-disconnected (HPD) ewe

AU - Smith, A. I.

AU - Wallace, C. A.

AU - Autelitano, D. J.

AU - Cheng, M. C.

AU - Clarke, I. J.

AU - Funder, J. W.

PY - 1986/4/11

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N2 - We have used specific radioimmunoassay and high-performance liquid chromatography (HPLC) to determine content and molecular forms of pituitary α-N-acetylated endorphin (NacEP) in the intact and hypothalamo-pituitary-disconnected (HPD) sheep. No significant differences were seen in anterior pituitary (AP) immunoreactive (ir-) NacEP levels (control=4.34±0.45 ng/mg; HPD=4.38±0.83 ng/mg; n=4) or in HPLC profiles following pituitary disconnection. In contrast, levels of ir-NacEP in the HPD intermediate lobe (IL) (263±16.5 ng/mg; n=4) were double those in the control (110±14 ng/mg; n=4), accompanied by a marked change in the molecular profile. In the control IL, the major species were NacαEP (30%), NacγEP (30%) and NacβEP1-27 (35%); in HPD IL, NacβEP1-27 (65%) is the dominant molecular form, with NacαEP (11%) and NacγEP (9%) relatively minor components.

AB - We have used specific radioimmunoassay and high-performance liquid chromatography (HPLC) to determine content and molecular forms of pituitary α-N-acetylated endorphin (NacEP) in the intact and hypothalamo-pituitary-disconnected (HPD) sheep. No significant differences were seen in anterior pituitary (AP) immunoreactive (ir-) NacEP levels (control=4.34±0.45 ng/mg; HPD=4.38±0.83 ng/mg; n=4) or in HPLC profiles following pituitary disconnection. In contrast, levels of ir-NacEP in the HPD intermediate lobe (IL) (263±16.5 ng/mg; n=4) were double those in the control (110±14 ng/mg; n=4), accompanied by a marked change in the molecular profile. In the control IL, the major species were NacαEP (30%), NacγEP (30%) and NacβEP1-27 (35%); in HPD IL, NacβEP1-27 (65%) is the dominant molecular form, with NacαEP (11%) and NacγEP (9%) relatively minor components.

KW - acetylation

KW - dopamine

KW - high-performance liquid chromatography

KW - processing

KW - radioimmunoassay

KW - sheep pituitary

KW - β-endorphin

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ER -

α-N-acetylated β-endorphin is differentially processed in the normal and hypothalamo-pituitary-disconnected (HPD) ewe

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Keywords

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