α-LTX and α-LTXN4C induce [Ca2+] i elevation through different mechanisms in pancreatic β cells

Yaming Jiu, Zhitao Hu, Jie Liu, Zhengxin Wu, Tao Xu

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Abstract

α-latrotoxin (α-LTX), a neurotoxin from black-widow spider, causes vesicles release in presynapse of nerve terminal after binding to specific membrane receptors. α-LTXN4C is an effective tool binding to calcium independent receptor for latrotoxin (CIRL), which is used to elucidate the mechanism of receptor-mediated signal pathway. In our study on the pancreatic β cells, we found that α-LTX inserts into the plasma membrane and forms stable non-selective cation channels. The influx of extracellular Ca2+ through the channels causes massive Ca 2+-dependent exocytosis of insulin-containing vesicles, whereas, α-LTXN4C, binding with its receptor CIRL in extracellular divalent cation-dependent way, increases [Ca2+]i by mobilization of the intracellular calcium stores.

Original languageEnglish
Pages (from-to)158-163
Number of pages6
JournalChinese Science Bulletin
Volume51
Issue number2
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • α-Latrotoxin
  • α-LTX
  • Calcium

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