Uncovering microRNA decay regulation in mammalian cells

MicroRNAs (miRNAs) constitute a novel mechanism used by cells to regulate gene expression. However,
very little is known about the mechanisms affecting miRNA accumulation. Characterisation of the kinetics of
miRNA turnover is of paramount importance to establish the reliability of miRNAs as novel biomarkers. Here
I propose to characterise miRNA stability in mammalian cells, investigate mechanisms of turnover and
establish their importance on the regulatory function of miRNAs. Such information is critical in the future
development of targeted therapeutics.