The phosphoinositide PtdIns(4,5)P2 plays a critical role in regulating vesicular trafficking, gene transcription and the actin cytoskeleton. We have cloned and characterized a family of enzymes from Saccharomyces cerevisiae known as 5-phosphatases which terminate the signals generated by this phosphoinositide. We aim to investigate how the 5-phosphatases regulate the spatial and temporal distribution of PtdIns(4,5)P2 on intracellular membranes as well as determining the role of this phosphoinositide in vacuole function, endocytosis and transcription. Collectively, these studies will provide novel information about the functionally significant signalling pathways regulated by this important phosphoinositide.
|Effective start/end date||3/01/06 → 31/12/08|
- Australian Research Council (ARC): AUD260,000.00